Abstract: The transcription factor GhABF2 is homologous to AREB1/ABF2. The drought resistance of plants are improved by overexpressing GhABF2. In order to create the drought-resistant germplasm resources of potato, GhABF2 gene was transferred into potato plantlet of cultivar “Atlantic” and mediated by Agrobacterium tumefaciens and 12 lines of kan-resistant seedlings were obtained. By using PCR、RT-PCR and Southern-blotting, this paper screened out eight transgenic lines with high GhABF2 expression, and investigated their physiological biochemical indexes and growth characteristics under drought conditions. The results showed that the biomass, chlorophyll content, soluble sugar content, proline content,  superoxide dismutase (SOD) and peroxidase (POD) activities of transgenic plants were significantly higher than those of the control under PEG-6000 stress. It was suggested that the drought tolerance of potato was improved by overexpressing GhABF2. The present research provided important genetic resources for drought-tolerant new variety breeding, and had important theoretical and practical significance.

Key words: potato, GhABF2, transgenetic, drought tolerance

摘要： 转录因子GhABF2是AREB1/ABF2的同源基因，过表达GhABF2能显著提高植物的抗旱性。为了创制马铃薯抗旱种质资源，利用根癌农杆菌介导法将GhABF2基因导入马铃薯栽培品种‘大西洋’中，获得卡那霉素抗性苗12株，并通过PCR、qRT-PCR、Southern-blotting检测，筛选出GhABF2基因整合到受体基因组且表达量较高的8个转基因株系。在干旱条件下，对苗期植株生长情况及相关生理生化指标进行测定，结果表明:在PEG模拟干旱胁迫下,转基因株系与对照植株相比，其生物量显著增加，叶绿素、可溶性糖和脯氨酸含量升高，超氧化物歧化酶（superoxide dismutase，SOD）和过氧化物酶（peroxidase，POD）活性显著提高，转GhABF2基因植株在生长状态、生理生化指标方面都表现出更强的耐受性，说明转录因子GhABF2的高表达显著提高了马铃薯的抗旱性。结果为马铃薯抗旱新品种的选育提供中间材料和重要基因资源，具有重要的理论和实际意义。

关键词: 马铃薯, GhABF2, 转基因, 抗旱性