Abstract:

Enzymes as natural biocatalysts with a high catalytic efficiency and substrate\|selected specificity characteristics, have important application value in industrial and agricultural production. The transformation of the zymoprotien is an important source to obtain excellent zymoprotien. In this paper, the strategy and methods for improving enzyme heat and pH stability, remolding protease resistance, and reforming multi\|characters of zymoprotein were reviewed, aiming at providing references for related experiments and studies.

Key words: enzyme, molecular design, thermo\, stability, pH stability, protease resistance, glycosylation

摘要：

酶作为天然的生物催化剂，具有高催化效率和高底物选择特异性的特点，在工农业生产上有重要的应用价值。通过对酶蛋白的改造是获得优良性质酶蛋白的重要来源。针对饲用酶应用的特点，从酶蛋白理性分子设计策略及其技术方法角度综述了酶的热稳定性及pH稳定性改造，蛋白酶抗性改造和酶蛋白的多性质改造等，以期为相关实验研究提供技术参考。

关键词: 酶；分子设计；热稳定性；pH稳定性；蛋白酶抗性；糖基化