Several Major RACE Technologies and their Application

doi:10.3969/j.issn.1008-0864.2012.02.13

›› 2012, Vol. 14 ›› Issue (2): 81-87.DOI: 10.3969/j.issn.1008-0864.2012.02.13

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Several Major RACE Technologies and their Application

XU Ye1, LIU Ya-ting1,2, DAI Wen-qiong3, ZHU Xiao-yuan1, CHEN Xue-jiao1, WU Fa-jing1, ZHI Long1, WANG Man-jun1,2

(1.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201|2.Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University,
Hubei Huanggang 438000|3.Science and Technology Training Center of Kunming, Kunming 650021, China)

Received:2011-10-11 Revised:2012-02-04 Online:2012-04-15 Published:2012-04-13

几种主要的RACE技术及应用

徐烨1,刘雅婷1,2,代文琼3,朱晓媛1,陈雪娇1,仵发静1,智龙1,王曼君1,2

(1.云南农业大学农学与生物技术学院, 昆明 650201|2.黄冈师范学院经济林木种质改良与资源综合利用湖北省重点实验室, 湖北黄冈 438000|3.昆明市科技培训中心, 昆明 650021)

通讯作者: 刘雅婷,教授,博士,研究方向为植物病原微生物与寄主互作关系研究。 Tel:86-871-5227726; E-mail:liuyating999@yahoo.com.cn
作者简介:徐烨,硕士研究生,研究方向为植物病毒的分子鉴定。E-mail: crafy@qq.com。
基金资助:
国家自然科学基金项目(30960224);经济林木种质改良与资源综合利用湖北省重点实验室开放基金项目(20011BLK244)资助。

Abstract

Abstract:

Rapid Amplification of cDNA Ends (RACE) is a kind of technology, which can rapidly amplify mRNA ends with advantages of rapid, stable and high-success-rate. This paper expounded the principle of classic RACE, Adapter-Ligated RACE, RLM-RACE, Cap-switching RACE, cRACE, RCA-RACE, and their advantages and disadvantages, and their application at present. The paper also put forward suggestions and foreground for RACE application, so as to provide reference for scientists to choose the most appropriate RACE technology.

Key words: RACE, principle, application

摘要：

cDNA末端快速扩增(Rapid Amplification of cDNA Ends, RACE)是一种对mRNA进行末端快速扩增的技术，具有快速、稳定、成功率高等优点。阐述了经典RACE、Adapter-Ligated RACE、RLM-RACE、Cap-switching RACE、环形RACE和RCA-RACE的原理、优缺点及目前的应用，并提出了应用RACE技术的建议及RACE技术的应用前景，为实验人员选择最合适的RACE技术提供参考。

关键词: cDNA末端快速扩增技术;原理;应用

CLC Number:

XU Ye1, LIU Ya-ting1,2, DAI Wen-qiong3, ZHU Xiao-yuan1, CHEN Xue-jiao1, WU Fa-ji. Several Major RACE Technologies and their Application[J]. , 2012, 14(2): 81-87.

徐烨1,刘雅婷1,2,代文琼3,朱晓媛1,陈雪娇1,仵发静1,智龙1,王曼君1,2. 几种主要的RACE技术及应用[J]. , 2012, 14(2): 81-87.

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Several Major RACE Technologies and their Application

几种主要的RACE技术及应用

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