Abstract:

In order to discuss the effect of low dose and long duration of formalin on fish and recirculating aquaculture system(RAS), European seabass (Dicentrarchus labrax) was taken as material to study in RAS. European seabass was treated with 4 formalin concentration (0 mg/L, 30 mg/L, 60 mg/L and 90 mg/L). The results showed that the survival rate (98%) and WGR (350.26%) of European seabass had no significant difference (P>0.05) compared with the control group after being treated with 30 mg/L formalin. The 90 mg/L formalin treatment could significantly weaken the removal rate of nitrite. The activity of intestinal AMS and trypsin in stomach was significantly (P<0.05) increased in European seabass which was treated with 30 mg/L formalin. The 60 mg/L formalin treatment could damnify fish liver. European seabass suffered mild adversity stress after being treated with 60 mg/L and 90 mg/L formalin. The treatment of 90 mg/L formalin decreased the BAFs efficiency in wiping out nitrite (P<0.05), and the impact on removing ammonium was delayed compared with that on removing nitrite.

Key words: formalin, European seabass, RAS, physiological and biochemical index, BAF

摘要：

为探讨长时间低剂量福尔马林对鱼类及循环水养殖系统的影响,以欧鲈(Dicentrarchus labrax)为受试鱼在循环水养殖系统中进行实验。设置4个福尔马林浓度处理组(0 mg/L、30 mg/L、60 mg/L和90mg/L)进行分析,结果表明,30 mg/L福尔马林处理后,欧鲈存活率(98%)和增重率（350.26%）与对照组之间无显著性差异（P>0.05）;90 mg/L福尔马林处理使生物滤器去除亚硝酸盐氮效能产生明显削弱作用;30 mg/L福尔马林处理的欧鲈肠道内AMS和胃中的胰蛋白酶活性显著增加（P<0.05）。60 mg/L福尔马林处理使欧鲈肝脏遭到一定程度的损伤,且60 mg/L和90 mg/L福尔马林处理的欧鲈出现了轻度逆境胁迫。90 mg/L福尔马林处理的水体中亚硝酸盐氮的浓度升高,亚硝酸盐氮的去除率显著降低（P<0.05）,对去除氨氮的影响滞后于对去除亚硝酸盐氮的影响。

关键词: 福尔马林;欧鲈;循环水系统;生理生化指标;生物滤器