Optimization of Culture Medium of Chaetoceros muelleri by Response Surface Methodology

doi:10.13304/j.nykjdb.2022.0430

Abstract

Abstract:

In order to optimize the medium formula of Chaetoceros muelleri and improve its growth rate and cell density，response surface methodology was used to screen and verify the mass concentration of NaNO₃， NaH₂PO₄·H₂O， Na₂SiO₃ and C₆H₈FeNO₇ in f/2 medium. The results showed that， in accordance with single factor， the nutrient concentrations suitable for C. muelleri were screened， and the mass concentrations of NaNO₃， NaH₂PO₄·H₂O， Na₂SiO₃ and C₆H₈FeNO₇ were 10～30， 1.0～3.0， 20～80， 0.1～0.5 mg·L^-1， respectively. After being optimized by responding surface， the effects of four nutrient salts on the growth of C. muelleri were as follows： NaNO₃>Na₂SiO₃>NaH₂PO₄·H₂O>C₆H₈FeNO₇， and the optimal mass concentration of them were： NaNO₃ 21.8 mg·L^-1， NaH₂PO₄·H₂O 2.93 mg·L^-1， Na₂SiO₃ 54.2 mg·L^-1 and C₆H₈FeNO₇ 0.158 mg·L^-1. Cell density of C. muelleri in optimized culture medium group enhanced 27.95%， significantly higher than that of f/2 medium group （P<0.05）. At 4^th days， the maximum light conversion efficiency of photosynthetic system PSII in experimental group was significantly higher than that in control group （P<0.05）. To sum up， the selected nutrient mass concentration could significantly improve the growth rate and cell density of C. muelleri， which could provide data reference for high-density culture of C. muelleri.

Key words: Chaetoceros muelleri, response surface, nutrient, cell density

摘要：

为优化牟氏角毛藻的培养基配方，提高牟氏角毛藻生长速度及细胞密度，采用响应面法筛选f/2培养基中的NaNO₃、NaH₂PO₄·H₂O、Na₂SiO₃和C₆H₈FeNO₇质量浓度并进行验证。结果表明，根据单因素试验筛选出牟氏角毛藻相对适宜的营养盐质量浓度范围为：NaNO₃ 10～30 mg·L^-1，NaH₂PO₄·H₂O 1.0～3.0 mg·L^-1，Na₂SiO₃ 20～80 mg·L^-1，C₆H₈FeNO₇ 0.1～0.5 mg·L^-1；通过响应面优化，4种营养盐对牟氏角毛藻生长的影响强弱依次为NaNO₃>Na₂SiO₃>NaH₂PO₄·H₂O>C₆H₈FeNO₇，最佳质量浓度分别为NaNO₃ 21.8 mg·L^-1、NaH₂PO₄·H₂O 2.93 mg·L^-1、Na₂SiO₃ 54.2 mg·L^-1、C₆H₈FeNO₇ 0.158 mg·L^-1。使用优化后的培养基培养该藻，密度提高27.95%，显著高于f/2培养基组的细胞密度（P<0.05），第4天后试验组牟氏角毛藻光合系统PSⅡ最大光能转换效率显著高于对照组（P<0.05）。综上所述，该方法筛选得出的营养盐质量浓度可以显著提高牟氏角毛藻的生长速度及细胞密度，结果为牟氏角毛藻的高密度培养提供数据参考。

关键词: 牟氏角毛藻, 响应面法, 营养盐, 细胞密度

CLC Number:

S963.21⁺3

Qi LI, Shulin ZHANG, Dajuan ZHANG, Yingxuan JIA, Zebin WANG. Optimization of Culture Medium of Chaetoceros muelleri by Response Surface Methodology[J]. Journal of Agricultural Science and Technology, 2023, 25(8): 225-233.

李琦, 张树林, 张达娟, 贾滢暄, 王泽斌. 响应面法改良牟氏角毛藻培养基[J]. 中国农业科技导报, 2023, 25(8): 225-233.

Figures/Tables 11

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方差来源 Variance sources	平方和 Sum of squares	自由度 Degree of freedom	均方 Mean squares	F值 F value	P值 P value	显著性 Significance
模型Model	44 720.85	14	3 194.35	29.89	<0.000 1	**
A-A	11 797.01	1	11 797.01	110.39	<0.000 1	**
B-B	575.47	1	575.47	5.38	0.035 9	*
C-C	878.77	1	878.77	8.22	0.012 4	*
D-D	322.61	1	322.61	3.02	0.104 2
AB	258.41	1	258.41	2.42	0.142 3
AC	704.90	1	704.90	6.60	0.022 3	*
AD	12.74	1	12.74	0.119 3	0.735 0
BC	730.62	1	730.62	6.84	0.020 4	*
BD	1 021.76	1	1 021.76	9.56	0.008 0	**
CD	1 962.93	1	1 962.93	18.37	0.000 8	**
A²	22 529.99	1	22 529.99	210.81	<0.000 1	**
B²	1 679.08	1	1 679.08	15.71	0.001 4	**
C²	6 714.11	1	6 714.11	62.82	<0.000 1	**
D²	4 466.73	1	4 466.73	41.80	<0.000 1	**
残差Residual	1 496.19	14	106.87
失拟项Lack of fit	1 280.10	10	128.01	2.37	0.210 5
纯误差Pure error	216.09	4	54.02
总和 Cor total	46 217.04	28

方差来源 Variance sources	平方和 Sum of squares	自由度 Degree of freedom	均方 Mean squares	F值 F value	P值 P value	显著性 Significance
模型Model	44 720.85	14	3 194.35	29.89	<0.000 1	**
A-A	11 797.01	1	11 797.01	110.39	<0.000 1	**
B-B	575.47	1	575.47	5.38	0.035 9	*
C-C	878.77	1	878.77	8.22	0.012 4	*
D-D	322.61	1	322.61	3.02	0.104 2
AB	258.41	1	258.41	2.42	0.142 3
AC	704.90	1	704.90	6.60	0.022 3	*
AD	12.74	1	12.74	0.119 3	0.735 0
BC	730.62	1	730.62	6.84	0.020 4	*
BD	1 021.76	1	1 021.76	9.56	0.008 0	**
CD	1 962.93	1	1 962.93	18.37	0.000 8	**
A²	22 529.99	1	22 529.99	210.81	<0.000 1	**
B²	1 679.08	1	1 679.08	15.71	0.001 4	**
C²	6 714.11	1	6 714.11	62.82	<0.000 1	**
D²	4 466.73	1	4 466.73	41.80	<0.000 1	**
残差Residual	1 496.19	14	106.87
失拟项Lack of fit	1 280.10	10	128.01	2.37	0.210 5
纯误差Pure error	216.09	4	54.02
总和 Cor total	46 217.04	28

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