Abstract: Isobaric tags for relative and absolute quantitation (iTRAQ) is a novel high-throughput technique for protein sequencing. This technology combined with high-resolution mass spectrometer is a powerful means for carrying out qualitative and quantitative analysis up to 8 samples simultaneously. This method can test wide range of protein with low limitation, and yet has reliabal and accurate results. Recently, iTRAQ technology has been widely used in studying plant structure, function and expression, etc.. Rice (Oryza sativa L.) is a main grain crop for human, and also the first cereal model plant completed genome sequencing. It is of great significance to study rice growth, development and regulation mechanism at the proteome level. This paper summarized the principle, operation procedure, advantages and disadvantages of iTRAQ technology, and focused on analyzing rice proteomics research in plant tissue and organ, subcellular level, environment stress, hormone induce, and rice mutant, etc.. Finally this paper prospected the application of iTRAQ technology in rice proteomics.

Key words: isobaric tags for relative and absolute quantitation (iTRAQ), rice, proteomics, stress, hormone, mutant

摘要： 同位素标记相对和绝对定量技术（iTRAQ）是一种新型的高通量的蛋白质测序技术，其通过与高精度的质谱仪串联，可同时对多达8个样品进行定性与定量分析，测定蛋白范围广泛、检测限低、分析结果可靠、精度高；目前已广泛应用于植物体的结构、功能、表达等方面的研究。水稻是人类的主要粮食作物，也是首个完成基因组测序的谷类模式植物，在蛋白质组水平上研究水稻的生长发育、调控机制具有重 要意义。综述了iTRAQ的原理、操作流程和优缺点，重点就iTRAQ技术在水稻组织器官、亚细胞水平、逆境胁迫、激素诱导、突变体等方面的蛋白质组学研究进行了分析，并对iTRAQ技术在水稻蛋白质组学上的应用研究进行了展望。

关键词: 同位素标记相对和绝对定量技术（iTRAQ）, 水稻, 蛋白质组学, 胁迫, 激素, 突 变体