Abstract: This paper screened 3 preparation methods of tobacco chlorogenic acid (CA) nanoliposome, and analyzed their stability. Dialysis method was employed to determine entrapment efficiency (EE) of chlorogenic acid liposomes. Thin film hydration, reverse phase evaporation technique, and ethyl alcohol injection methods were screened based on EE and diameter of liposomes. The effects of film (lecithin+cholesterol)/CA mass ratio, lecithin/cholesterol mass ratio and ultrasonication time on EE and effective loading ability of liposomes were investigated. Orthogonal combination design was used to determine the best process parameters. Finally, the stability of the prepared liposomes was evaluated. The results showed that when film/CA mass ratio was 9∶1, lecithin/cholesterol mass ratio was 4∶1 and ultrasonication time was 5 min, the CA nanoliposomes showed regular spherical. Its average grain diameter was 77.16 nm with good dispersibility. The embedding rate and efficient loading capacity could be up to 88.02% and 9.69%, respectively. Post-embedding liposome thermal and alkaline stabilities were all improved. UV test showed that CA was encapsulated successfully into nanoliposomes. Above results showed that CA nanoliposomes prepared by ethyl alcohol injection method had small grain diameter, good dispersion, better thermal stability, alkaline stability and storage stability.

Key words: tobacco chlorogenic acid, lipidosome, preparation technics, stability

摘要： 筛选了烟草绿原酸纳米脂质体的3种制备方法并研究其稳定性。以透析法测定烟草绿原酸脂质体的包埋率；以包埋率和粒径为主要指标筛选薄膜分散法、乙醇注入结合超声法和逆相蒸发法3种制备方法；考察蛋黄卵磷脂与绿原酸的比例、蛋黄卵磷脂与胆固醇的比例及超声时间对绿原酸纳米脂质体包埋率和有效载量的影响；以正交组合设计试验确定最佳工艺参数；最后对最佳条件下制备的脂质体稳定性进行评价。结果表明：蛋黄卵磷脂与绿原酸质量比9∶1、卵磷脂与胆固醇质量比4∶1、实际超声时间5 min所制备的烟草绿原酸纳米脂质体呈球形，平均粒径为77.16 nm，分散性良好，包埋率和有效载量最高分别达到88.02%和9.69%；包埋后的脂质体热稳定性和碱性稳定性均得到提高；紫外测试表明绿原酸被成功包覆在脂质体中。上述结果表明乙醇注入结合超声法制备的绿原酸纳米脂质体粒径小、分散性好，具有良好的热稳定性、碱性稳定性和贮藏稳定性。

关键词: 烟草绿原酸, 脂质体, 制备工艺, 稳定性