








中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (11): 56-63.DOI: 10.13304/j.nykjdb.2025.0574
周良星1,2(
), 赵敏1,2,3(
), 舒斌4, 吴坤岚4, 席庆凯2, 任宣宇2, 陈颖4(
), 刘建高1,2(
)
收稿日期:2025-08-05
接受日期:2025-09-04
出版日期:2025-11-15
发布日期:2025-11-17
通讯作者:
陈颖,刘建高
作者简介:周良星 E-mail:zlx19880909@163.com基金资助:
Liangxing ZHOU1,2(
), Min ZHAO1,2,3(
), Bin SHU4, Kunlan WU4, Qingkai XI2, Xuanyu REN2, Ying CHEN4(
), Jiangao LIU1,2(
)
Received:2025-08-05
Accepted:2025-09-04
Online:2025-11-15
Published:2025-11-17
Contact:
Ying CHEN,Jiangao LIU
摘要:
为探讨在凡纳滨对虾(Litopenaeus vannamei)饲料中添加辅酶Q10蛋白替代鱼粉并补充牛磺酸对其生长及抗应激能力的影响,开展为期56 d的养殖试验。以鱼粉饲料为对照(CK),分别设置5%辅酶Q10蛋白(Q1)、5%辅酶Q10蛋白+0.3%牛磺酸(Q2)、5%辅酶Q10蛋白+0.6%牛磺酸(Q3)、10%辅酶Q10蛋白(Q4)、10%辅酶Q10蛋白+0.3%牛磺酸(Q5)及10%辅酶Q10蛋白+0.6%牛磺酸(Q6),共计7个处理。结果表明,Q3处理特定生长率高于其他处理,且饵料系数显著低于其他处理;辅酶Q10蛋白处理运输应激后的成活率均高于CK,但处理之间差异不显著;运输应激后,添加牛磺酸处理血浆的超氧化物歧化酶活性、总抗氧化能力高于CK,而丙二醛含量低于CK,但差异不显著。综上所述,在该研究条件下,在饲料中添加5%辅酶Q10蛋白和0.6%牛磺酸替代鱼粉,可以提高凡纳滨对虾的生长性能及抗应激能力。研究结果揭示了辅酶Q10蛋白和牛磺酸在提高对虾养殖效益和可持续发展中的潜力,为凡纳滨对虾低鱼粉饲料的开发提供了科学依据。
中图分类号:
周良星, 赵敏, 舒斌, 吴坤岚, 席庆凯, 任宣宇, 陈颖, 刘建高. 辅酶Q10蛋白和牛磺酸替代鱼粉对凡纳滨对虾生长及抗应激能力的影响[J]. 中国农业科技导报, 2025, 27(11): 56-63.
Liangxing ZHOU, Min ZHAO, Bin SHU, Kunlan WU, Qingkai XI, Xuanyu REN, Ying CHEN, Jiangao LIU. Effect of Coenzyme Q10 Protein and Taurine Replacing Fish Meal on Growth and Anti-stress Ability of Litopenaeus vannamei[J]. Journal of Agricultural Science and Technology, 2025, 27(11): 56-63.
| 类型Type | 指标Index | CK | Q1 | Q2 | Q3 | Q4 | Q5 | Q6 |
|---|---|---|---|---|---|---|---|---|
| 配方 Ingredient | 鱼粉Fish meal | 29.00 | 23.50 | 23.50 | 23.50 | 18.00 | 18.00 | 18.00 |
| 辅酶Q10蛋白CoQ10 protein | 0.00 | 5.00 | 5.00 | 5.00 | 10.00 | 10.00 | 10.00 | |
| 牛磺酸Taurine | 0.00 | 0.00 | 0.30 | 0.60 | 0.00 | 0.30 | 0.60 | |
| 虾粉Shrimp meal | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 | |
| 鸡肉粉Poultry powder | 8.50 | 8.50 | 8.50 | 8.50 | 8.50 | 8.50 | 8.50 | |
| 虾膏Shrimp paste | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | |
| 鱼溶浆Fish soluble | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | |
| 酵母水解物Yeast hydrolyte | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | |
| 花生饼Peanut cake | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 | |
| 豆粕Soybean meal | 11.85 | 11.85 | 11.85 | 11.85 | 11.85 | 11.85 | 11.85 | |
| 高筋面粉Gluten flour | 20.50 | 20.50 | 20.50 | 20.50 | 20.50 | 20.50 | 20.50 | |
| 大豆磷脂油Soybean-lecithin oil | 3.00 | 3.25 | 3.25 | 3.25 | 3.50 | 3.50 | 3.50 | |
| 膨润土Bentonite | 0.35 | 0.60 | 0.30 | 0.00 | 0.85 | 0.55 | 0.25 | |
| 磷酸二氢钙Ca(H2PO4)2 | 1.80 | 1.80 | 1.80 | 1.80 | 1.80 | 1.80 | 1.80 | |
| 预混料Premix | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | |
| 营养成分Nutritional ingredient | 粗蛋白Crude protein | 45.87 | 45.34 | 45.57 | 45.62 | 45.68 | 45.73 | 45.71 |
| 粗脂肪Crude lipid | 7.83 | 7.89 | 7.92 | 7.91 | 7.75 | 7.85 | 7.73 | |
| 粗灰分Crude ash | 11.61 | 11.54 | 11.48 | 11.71 | 11.34 | 11.27 | 11.42 | |
| 钙Ga | 2.18 | 2.17 | 1.85 | 1.99 | 1.71 | 1.87 | 1.89 | |
| 磷P | 1.82 | 1.79 | 1.62 | 1.77 | 1.8 | 1.72 | 1.72 | |
| 盐分NaCl | 0.89 | 0.48 | 0.51 | 1.14 | 0.97 | 1.12 | 1.09 | |
| 粗纤维Crude fiber | 1.84 | 1.31 | 2.13 | 2.72 | 9.44 | 8.66 | 7.55 | |
| 氨基酸总和Total amino acids | 38.94 | 39.44 | 40.08 | 38.99 | 40.06 | 39.25 | 39.54 | |
| 赖氨酸Lys | 2.80 | 2.64 | 2.70 | 2.59 | 2.49 | 2.50 | 2.54 | |
| 蛋氨酸Met | 0.92 | 0.92 | 0.93 | 0.88 | 0.95 | 0.93 | 0.94 | |
| 苏氨酸Thr | 1.71 | 1.73 | 1.76 | 1.71 | 1.78 | 1.73 | 1.73 | |
| 缬氨酸Val | 1.79 | 1.81 | 1.93 | 1.83 | 1.89 | 1.74 | 1.75 | |
| 亮氨酸Leu | 3.21 | 3.27 | 3.34 | 3.26 | 3.38 | 3.30 | 3.30 | |
| 异亮氨酸Ile | 1.40 | 1.37 | 1.49 | 1.41 | 1.42 | 1.30 | 1.31 | |
| 苯丙氨酸 Phe | 1.96 | 2.02 | 2.04 | 2.01 | 2.08 | 2.07 | 2.05 | |
| 精氨酸Arg | 2.75 | 2.81 | 2.87 | 2.80 | 2.91 | 2.81 | 2.84 | |
| 组氨酸His | 1.23 | 1.23 | 1.24 | 1.21 | 1.25 | 1.23 | 1.23 | |
| 天冬氨酸Asp | 4.22 | 4.25 | 4.29 | 4.18 | 4.35 | 4.30 | 4.32 | |
| 胱氨酸Cys | 0.48 | 0.49 | 0.49 | 0.48 | 0.48 | 0.47 | 0.49 | |
| 丙氨酸Ala | 2.64 | 2.77 | 2.78 | 2.73 | 2.89 | 2.87 | 2.88 | |
| 营养成分Nutritional ingredient | 甘氨酸Gly | 2.79 | 2.91 | 2.94 | 2.88 | 2.92 | 2.88 | 2.90 |
| 谷氨酸Glu | 6.87 | 6.90 | 6.94 | 6.80 | 6.90 | 6.82 | 6.85 | |
| 脯氨酸Pro | 2.15 | 2.26 | 2.29 | 2.20 | 2.30 | 2.25 | 2.34 | |
| 丝氨酸Ser | 2.02 | 2.06 | 2.05 | 2.02 | 2.07 | 2.05 | 2.07 |
表1 试验饲料配方组成和营养成分 (续表(Continued))
Table 1 (%)
| 类型Type | 指标Index | CK | Q1 | Q2 | Q3 | Q4 | Q5 | Q6 |
|---|---|---|---|---|---|---|---|---|
| 配方 Ingredient | 鱼粉Fish meal | 29.00 | 23.50 | 23.50 | 23.50 | 18.00 | 18.00 | 18.00 |
| 辅酶Q10蛋白CoQ10 protein | 0.00 | 5.00 | 5.00 | 5.00 | 10.00 | 10.00 | 10.00 | |
| 牛磺酸Taurine | 0.00 | 0.00 | 0.30 | 0.60 | 0.00 | 0.30 | 0.60 | |
| 虾粉Shrimp meal | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 | |
| 鸡肉粉Poultry powder | 8.50 | 8.50 | 8.50 | 8.50 | 8.50 | 8.50 | 8.50 | |
| 虾膏Shrimp paste | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | |
| 鱼溶浆Fish soluble | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | |
| 酵母水解物Yeast hydrolyte | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | |
| 花生饼Peanut cake | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 | 8.00 | |
| 豆粕Soybean meal | 11.85 | 11.85 | 11.85 | 11.85 | 11.85 | 11.85 | 11.85 | |
| 高筋面粉Gluten flour | 20.50 | 20.50 | 20.50 | 20.50 | 20.50 | 20.50 | 20.50 | |
| 大豆磷脂油Soybean-lecithin oil | 3.00 | 3.25 | 3.25 | 3.25 | 3.50 | 3.50 | 3.50 | |
| 膨润土Bentonite | 0.35 | 0.60 | 0.30 | 0.00 | 0.85 | 0.55 | 0.25 | |
| 磷酸二氢钙Ca(H2PO4)2 | 1.80 | 1.80 | 1.80 | 1.80 | 1.80 | 1.80 | 1.80 | |
| 预混料Premix | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | |
| 营养成分Nutritional ingredient | 粗蛋白Crude protein | 45.87 | 45.34 | 45.57 | 45.62 | 45.68 | 45.73 | 45.71 |
| 粗脂肪Crude lipid | 7.83 | 7.89 | 7.92 | 7.91 | 7.75 | 7.85 | 7.73 | |
| 粗灰分Crude ash | 11.61 | 11.54 | 11.48 | 11.71 | 11.34 | 11.27 | 11.42 | |
| 钙Ga | 2.18 | 2.17 | 1.85 | 1.99 | 1.71 | 1.87 | 1.89 | |
| 磷P | 1.82 | 1.79 | 1.62 | 1.77 | 1.8 | 1.72 | 1.72 | |
| 盐分NaCl | 0.89 | 0.48 | 0.51 | 1.14 | 0.97 | 1.12 | 1.09 | |
| 粗纤维Crude fiber | 1.84 | 1.31 | 2.13 | 2.72 | 9.44 | 8.66 | 7.55 | |
| 氨基酸总和Total amino acids | 38.94 | 39.44 | 40.08 | 38.99 | 40.06 | 39.25 | 39.54 | |
| 赖氨酸Lys | 2.80 | 2.64 | 2.70 | 2.59 | 2.49 | 2.50 | 2.54 | |
| 蛋氨酸Met | 0.92 | 0.92 | 0.93 | 0.88 | 0.95 | 0.93 | 0.94 | |
| 苏氨酸Thr | 1.71 | 1.73 | 1.76 | 1.71 | 1.78 | 1.73 | 1.73 | |
| 缬氨酸Val | 1.79 | 1.81 | 1.93 | 1.83 | 1.89 | 1.74 | 1.75 | |
| 亮氨酸Leu | 3.21 | 3.27 | 3.34 | 3.26 | 3.38 | 3.30 | 3.30 | |
| 异亮氨酸Ile | 1.40 | 1.37 | 1.49 | 1.41 | 1.42 | 1.30 | 1.31 | |
| 苯丙氨酸 Phe | 1.96 | 2.02 | 2.04 | 2.01 | 2.08 | 2.07 | 2.05 | |
| 精氨酸Arg | 2.75 | 2.81 | 2.87 | 2.80 | 2.91 | 2.81 | 2.84 | |
| 组氨酸His | 1.23 | 1.23 | 1.24 | 1.21 | 1.25 | 1.23 | 1.23 | |
| 天冬氨酸Asp | 4.22 | 4.25 | 4.29 | 4.18 | 4.35 | 4.30 | 4.32 | |
| 胱氨酸Cys | 0.48 | 0.49 | 0.49 | 0.48 | 0.48 | 0.47 | 0.49 | |
| 丙氨酸Ala | 2.64 | 2.77 | 2.78 | 2.73 | 2.89 | 2.87 | 2.88 | |
| 营养成分Nutritional ingredient | 甘氨酸Gly | 2.79 | 2.91 | 2.94 | 2.88 | 2.92 | 2.88 | 2.90 |
| 谷氨酸Glu | 6.87 | 6.90 | 6.94 | 6.80 | 6.90 | 6.82 | 6.85 | |
| 脯氨酸Pro | 2.15 | 2.26 | 2.29 | 2.20 | 2.30 | 2.25 | 2.34 | |
| 丝氨酸Ser | 2.02 | 2.06 | 2.05 | 2.02 | 2.07 | 2.05 | 2.07 |
处理 Treatment | 初均质量 Initial average weight/g | 末均质量 Final average weight/g | 摄食率 Feeding rate/% | 增重率 Weight gain rate/% | 特定生长率 Specific growth rate/(%·d-1) | 饵料系数 Feed coefficient |
|---|---|---|---|---|---|---|
| CK | 1.23±0.09 | 8.11±0.27 b | 3.98±0.04 b | 494.77±44.83 bc | 3.30±0.14 bc | 1.51±0.05 b |
| Q1 | 1.23±0.08 | 7.33±0.69 ab | 4.33±0.21 c | 437.77±28.95 ab | 3.11±0.10 ab | 1.71±0.08 cd |
| Q2 | 1.25±0.07 | 7.15±0.72 ab | 4.14±0.22 bc | 438.74±35.33 ab | 3.12±0.12 ab | 1.63±0.11 bcd |
| Q3 | 1.27±0.05 | 8.26±0.50 b | 3.63±0.12 a | 525.79±53.99 c | 3.39±0.16 c | 1.35±0.08 a |
| Q4 | 1.32±0.09 | 7.97±0.74 ab | 3.98±0.22 b | 436.03±14.45 ab | 3.11±0.05 ab | 1.57±0.08 bc |
| Q5 | 1.24±0.08 | 6.91±0.40 a | 4.39±0.09 c | 414.24±20.56 a | 3.03±0.07 a | 1.76±0.02 d |
| Q6 | 1.30±0.08 | 7.18±0.86 ab | 4.12±0.22 bc | 419.43±51.03 a | 3.04±0.18 a | 1.65±0.14 bcd |
表2 不同处理下凡纳滨对虾的生长性能和饲料效率
Table 2 Growth performance and feed efficiency of Litopenaeus vannamei under different treatments
处理 Treatment | 初均质量 Initial average weight/g | 末均质量 Final average weight/g | 摄食率 Feeding rate/% | 增重率 Weight gain rate/% | 特定生长率 Specific growth rate/(%·d-1) | 饵料系数 Feed coefficient |
|---|---|---|---|---|---|---|
| CK | 1.23±0.09 | 8.11±0.27 b | 3.98±0.04 b | 494.77±44.83 bc | 3.30±0.14 bc | 1.51±0.05 b |
| Q1 | 1.23±0.08 | 7.33±0.69 ab | 4.33±0.21 c | 437.77±28.95 ab | 3.11±0.10 ab | 1.71±0.08 cd |
| Q2 | 1.25±0.07 | 7.15±0.72 ab | 4.14±0.22 bc | 438.74±35.33 ab | 3.12±0.12 ab | 1.63±0.11 bcd |
| Q3 | 1.27±0.05 | 8.26±0.50 b | 3.63±0.12 a | 525.79±53.99 c | 3.39±0.16 c | 1.35±0.08 a |
| Q4 | 1.32±0.09 | 7.97±0.74 ab | 3.98±0.22 b | 436.03±14.45 ab | 3.11±0.05 ab | 1.57±0.08 bc |
| Q5 | 1.24±0.08 | 6.91±0.40 a | 4.39±0.09 c | 414.24±20.56 a | 3.03±0.07 a | 1.76±0.02 d |
| Q6 | 1.30±0.08 | 7.18±0.86 ab | 4.12±0.22 bc | 419.43±51.03 a | 3.04±0.18 a | 1.65±0.14 bcd |
处理 Treatment | 运输应激前成活率 Survival rate before transport stress | 运输应激后成活率 Survival rate after transport stress |
|---|---|---|
| CK | 0.90±0.04 | 0.63±0.08 |
| Q1 | 0.90±0.05 | 0.68±0.10 |
| Q2 | 0.94±0.04 | 0.72±0.08 |
| Q3 | 0.96±0.01 | 0.73±0.14 |
| Q4 | 0.89±0.11 | 0.70±0.09 |
| Q5 | 0.93±0.08 | 0.73±0.12 |
| Q6 | 0.94±0.07 | 0.75±0.15 |
表3 不同处理下凡纳滨对虾运输应激前后的成活率 (%)
Table 3 Survival rate of Litopenaeus vannamei under different treatments before and after transportation stress
处理 Treatment | 运输应激前成活率 Survival rate before transport stress | 运输应激后成活率 Survival rate after transport stress |
|---|---|---|
| CK | 0.90±0.04 | 0.63±0.08 |
| Q1 | 0.90±0.05 | 0.68±0.10 |
| Q2 | 0.94±0.04 | 0.72±0.08 |
| Q3 | 0.96±0.01 | 0.73±0.14 |
| Q4 | 0.89±0.11 | 0.70±0.09 |
| Q5 | 0.93±0.08 | 0.73±0.12 |
| Q6 | 0.94±0.07 | 0.75±0.15 |
分组 Group | 处理 Treatment | 总抗氧化能力T-AOC/(U·mg-1 prot) | 肝蛋白liver protein/(mg prot·mL-1) | 超氧化物歧化酶SOD/(U·mg-1 prot) | 丙二醛MDA/ (nmol·mg-1 prot) |
|---|---|---|---|---|---|
运输应激前 Before transport stress | CK | 0.48±0.29 | 4.27±0.48 | 11.52±0.88 | 2.93±1.00 |
| Q1 | 0.46±0.02 | 3.97±0.13 | 10.99±0.40 | 2.77±0.51 | |
| Q2 | 0.45±0.28 | 4.20±0.58 | 9.55±2.99 | 2.83±0.77 | |
| Q3 | 0.44±0.15 | 4.47±0.11 | 9.30±1.30 | 2.39±0.60 | |
| Q4 | 0.52±0.13 | 3.93±0.12 | 9.00±2.01 | 2.91±0.24 | |
| Q5 | 0.47±0.21 | 3.97±0.61 | 10.73±3.69 | 2.57±1.19 | |
| Q6 | 0.39±0.18 | 4.10±0.31 | 8.83±1.14 | 2.34±0.90 | |
运输应激后 After transport stress | CK | 0.43±0.23 | 4.21±0.10 | 7.16±0.29 | 2.44±0.37 |
| Q1 | 0.37±0.11 | 4.30±0.20 | 6.81±1.16 | 2.17±0.21 | |
| Q2 | 0.51±0.26 | 4.09±0.51 | 7.61±2.56 | 2.59±0.88 | |
| Q3 | 0.48±0.03 | 3.63±0.17 | 11.03±0.68 | 2.23±0.20 | |
| Q4 | 0.37±0.09 | 3.93±0.62 | 9.00±2.98 | 2.43±0.73 | |
| Q5 | 0.48±0.10 | 3.97±0.41 | 9.29±2.95 | 1.99±0.25 | |
| Q6 | 0.38±0.13 | 3.79±0.39 | 8.86±3.36 | 1.79±0.30 |
表4 不同处理下凡纳滨对虾运输应激前后肝胰腺的生化指标
Table 4 Biochemical indexes of hepatopancreas of Litopenaeus vannamei under different treatments before and after transportation stress
分组 Group | 处理 Treatment | 总抗氧化能力T-AOC/(U·mg-1 prot) | 肝蛋白liver protein/(mg prot·mL-1) | 超氧化物歧化酶SOD/(U·mg-1 prot) | 丙二醛MDA/ (nmol·mg-1 prot) |
|---|---|---|---|---|---|
运输应激前 Before transport stress | CK | 0.48±0.29 | 4.27±0.48 | 11.52±0.88 | 2.93±1.00 |
| Q1 | 0.46±0.02 | 3.97±0.13 | 10.99±0.40 | 2.77±0.51 | |
| Q2 | 0.45±0.28 | 4.20±0.58 | 9.55±2.99 | 2.83±0.77 | |
| Q3 | 0.44±0.15 | 4.47±0.11 | 9.30±1.30 | 2.39±0.60 | |
| Q4 | 0.52±0.13 | 3.93±0.12 | 9.00±2.01 | 2.91±0.24 | |
| Q5 | 0.47±0.21 | 3.97±0.61 | 10.73±3.69 | 2.57±1.19 | |
| Q6 | 0.39±0.18 | 4.10±0.31 | 8.83±1.14 | 2.34±0.90 | |
运输应激后 After transport stress | CK | 0.43±0.23 | 4.21±0.10 | 7.16±0.29 | 2.44±0.37 |
| Q1 | 0.37±0.11 | 4.30±0.20 | 6.81±1.16 | 2.17±0.21 | |
| Q2 | 0.51±0.26 | 4.09±0.51 | 7.61±2.56 | 2.59±0.88 | |
| Q3 | 0.48±0.03 | 3.63±0.17 | 11.03±0.68 | 2.23±0.20 | |
| Q4 | 0.37±0.09 | 3.93±0.62 | 9.00±2.98 | 2.43±0.73 | |
| Q5 | 0.48±0.10 | 3.97±0.41 | 9.29±2.95 | 1.99±0.25 | |
| Q6 | 0.38±0.13 | 3.79±0.39 | 8.86±3.36 | 1.79±0.30 |
分组 Group | 处理 Treatment | 总抗氧化能力 T-AOC/(mmol·L-1) | 超氧化物歧化酶SOD/(U·mg-1 prot) | 丙二醛MDA/(nmol·mg-1 prot) | 碱性磷酸酶AKP/(king U·100 mL-1) | 谷丙转氨酶ALT/(U·L-1) | 谷草转氨酶AST/(U·L-1) |
|---|---|---|---|---|---|---|---|
| 运输应激前Before transport stress | CK | 23.23±2.96 | 1 372.36±355.70 | 2.50±0.44 | 1.73±0.27 a | 5.24±0.97 | 2.93±0.90 |
| Q1 | 21.66±0.60 | 1 296.45±225.22 | 3.17±0.44 | 1.50±0.20 ab | 3.99±0.40 | 1.90±0.00 | |
| Q2 | 22.70±1.39 | 1 236.52±199.33 | 3.22±0.25 | 1.36±0.27 ab | 4.22±1.55 | 2.49±1.28 | |
| Q3 | 24.24±2.86 | 1 280.47±275.69 | 3.28±0.54 | 1.27±0.14 b | 3.07±0.48 | 1.71±0.94 | |
| Q4 | 22.14±0.61 | 1 172.60±36.62 | 3.33±0.67 | 1.42±0.10 ab | 4.47±0.84 | 2.69±0.66 | |
| Q5 | 23.03±1.79 | 1 218.55±165.36 | 2.94±0.35 | 1.27±0.31 b | 4.58±1.78 | 2.96±2.23 | |
| Q6 | 22.87±3.77 | 1 202.56±233.05 | 2.89±0.51 | 1.16±0.06 b | 4.58±1.75 | 2.99±1.37 | |
| 运输应激后After transport stress | CK | 1.10±0.37 | 330.01±34.93 | 5.89±1.18 | 1.64±0.28 ab | 3.40±1.85 | 5.35±0.82 |
| Q1 | 1.05±0.36 | 344.39±13.63 | 6.11±0.67 | 1.54±0.08 ab | 2.45±0.64 | 4.52±0.62 | |
| Q2 | 1.15±0.52 | 356.37±63.53 | 5.56±0.84 | 1.47±0.24 b | 3.01±0.50 | 4.99±0.99 | |
| Q3 | 1.41±0.13 | 364.36±73.14 | 5.17±0.44 | 1.29±0.14 b | 2.46±0.93 | 4.07±0.43 | |
| Q4 | 1.07±0.13 | 344.39±113.82 | 5.78±1.25 | 1.89±0.12 a | 2.72±1.22 | 3.87±0.77 | |
| Q5 | 2.22±1.15 | 375.55±32.55 | 4.72±0.67 | 1.88±0.29 a | 2.51±0.65 | 3.82±0.69 | |
| Q6 | 1.57±1.10 | 388.34±58.57 | 4.50±0.60 | 1.64±0.10 ab | 1.90±0.18 | 3.83±1.05 |
表5 不同处理下凡纳滨对虾运输应激前后的血浆生化指标
Table 5 Plasma biochemical indexes of Litopenaeus vannamei under different treatments before and after transportation stress
分组 Group | 处理 Treatment | 总抗氧化能力 T-AOC/(mmol·L-1) | 超氧化物歧化酶SOD/(U·mg-1 prot) | 丙二醛MDA/(nmol·mg-1 prot) | 碱性磷酸酶AKP/(king U·100 mL-1) | 谷丙转氨酶ALT/(U·L-1) | 谷草转氨酶AST/(U·L-1) |
|---|---|---|---|---|---|---|---|
| 运输应激前Before transport stress | CK | 23.23±2.96 | 1 372.36±355.70 | 2.50±0.44 | 1.73±0.27 a | 5.24±0.97 | 2.93±0.90 |
| Q1 | 21.66±0.60 | 1 296.45±225.22 | 3.17±0.44 | 1.50±0.20 ab | 3.99±0.40 | 1.90±0.00 | |
| Q2 | 22.70±1.39 | 1 236.52±199.33 | 3.22±0.25 | 1.36±0.27 ab | 4.22±1.55 | 2.49±1.28 | |
| Q3 | 24.24±2.86 | 1 280.47±275.69 | 3.28±0.54 | 1.27±0.14 b | 3.07±0.48 | 1.71±0.94 | |
| Q4 | 22.14±0.61 | 1 172.60±36.62 | 3.33±0.67 | 1.42±0.10 ab | 4.47±0.84 | 2.69±0.66 | |
| Q5 | 23.03±1.79 | 1 218.55±165.36 | 2.94±0.35 | 1.27±0.31 b | 4.58±1.78 | 2.96±2.23 | |
| Q6 | 22.87±3.77 | 1 202.56±233.05 | 2.89±0.51 | 1.16±0.06 b | 4.58±1.75 | 2.99±1.37 | |
| 运输应激后After transport stress | CK | 1.10±0.37 | 330.01±34.93 | 5.89±1.18 | 1.64±0.28 ab | 3.40±1.85 | 5.35±0.82 |
| Q1 | 1.05±0.36 | 344.39±13.63 | 6.11±0.67 | 1.54±0.08 ab | 2.45±0.64 | 4.52±0.62 | |
| Q2 | 1.15±0.52 | 356.37±63.53 | 5.56±0.84 | 1.47±0.24 b | 3.01±0.50 | 4.99±0.99 | |
| Q3 | 1.41±0.13 | 364.36±73.14 | 5.17±0.44 | 1.29±0.14 b | 2.46±0.93 | 4.07±0.43 | |
| Q4 | 1.07±0.13 | 344.39±113.82 | 5.78±1.25 | 1.89±0.12 a | 2.72±1.22 | 3.87±0.77 | |
| Q5 | 2.22±1.15 | 375.55±32.55 | 4.72±0.67 | 1.88±0.29 a | 2.51±0.65 | 3.82±0.69 | |
| Q6 | 1.57±1.10 | 388.34±58.57 | 4.50±0.60 | 1.64±0.10 ab | 1.90±0.18 | 3.83±1.05 |
| [1] | TACON A G J, METIAN M. Global overview on the use of fish meal and fish oil in industrially compounded aquafeeds: trends and future prospects [J]. Aquaculture, 2008, 285(l-4): l46-l58. |
| [2] | LIAO Z, GONG Y, WANG Z, et al.. Effects of dietary Rhodobacter Sphaeroides protein substitution of fishmeal and coenzyme Q10 supplementation on growth performance, intestinal microbiota and stress tolerance of litopenaeus vannamei in acute low salinity [J/OL]. Front. Mar. Sci., 2022, 9: 851649 [2025-06-20]. . |
| [3] | CRANE F L. Biochemical functions of coenzyme Q10 [J]. J. Am. Coll. Nutr., 2001, 20(6): 591-598. |
| [4] | ASGARI H, JALALI S M A L, FAGHANI M, et al.. Effects of dietary supplementation of coenzyme Q10 on growth performance, biochemical and physiological attributes of rainbow trout (Oncorhynchus mykiss) [J/OL]. Vet. Med. Sci., 2025, 11(3): e70324 [2025-06-20]. . |
| [5] | BASUINI M F, SHAHIN S A, TEIBA I I, et al.. The influence of dietary coenzyme Q10 and vitamin C on the growth rate, immunity, oxidative-related genes, and the resistance against Streptococcus agalactiae of Nile Tilapia (Oreochromis niloticus) [J/OL]. Aquaculture, 2021, 531: 735862 [2025-06-20]. . |
| [6] | HUXTABLE R J. Physiological actions of taurine [J]. Physiol. Rev., 1992, 72(1): 101-163. |
| [7] | WANG F R, DONG X F, TONG J M, et al.. Effects of dietary taurine supplementation on growth performance and immune status in growing Japanese quail (Coturnix coturnix Japonica) [J]. Poult. Sci., 2009, 88(7): 1394-1398. |
| [8] | ZHANG J, HU Y, AI Q, et al.. Effect of dietary taurine supplementation on growth performance, digestive enzyme activities and antioxidant status of juvenile black carp (Mylopharyngodon piceus) fed with low fish meal diet [J]. Aquac. Res., 2018, 49(9): 3187-3195. |
| [9] | HU Y, YANG G, LI Z, et al.. Effect of dietary taurine supplementation on growth, digestive enzyme, immunity and resistant to dry stress of rice field eel (Monopterus albus) fed low fish meal diets [J]. Aquac. Res., 2018, 49(6): 2108-2118. |
| [10] | KHAOIAN P, NGUYEN H P, OGITA Y, et al.. Taurine supplementation and palm oil substitution in low-fish meal diets for young yellowtail Seriola quinqueradiata [J]. Aquaculture, 2014, 420: 219-224. |
| [11] | WANG G, PENG K, HU J, et al.. Evaluation of defatted black soldier fly (Hermetia illucens L.) larvae meal as an alternative protein ingredient for juvenile Japanese seabass (Lateolabrax japonicus) diets [J]. Aquaculture, 2019, 507: 144-154. |
| [12] | WANG H, HU X, ZHENG Y, et al.. Effects of replacing fish meal with cottonseed protein concentrate on the growth, immune responses, digestive ability and intestinal microbial flora in Litopenaeus vannamei [J]. Fish Shellfish Immunol., 2022, 128: 91-100. |
| [13] | BAI F, NIU X, WANG X, et al.. Growth performance, biochemical composition and expression of lipid metabolism related genes in groupers (Epinephelus coioides) are altered by dietary taurine [J]. Aquac. Nutr., 2021, 27(6): 2690-2702. |
| [14] | MARTINS N, DIÓGENES A F, MAGALHÃES R, et al.. Dietary taurine supplementation affects lipid metabolism and improves the oxidative status of European seabass (Dicentrarchus labrax) juveniles [J/OL]. Aquaculture, 2021, 531: 735820 [2025-06-20]. . |
| [15] | MAI H, LI Y, SONG Z, et al.. Effect of taurine on growth and immune response of Pacific white shrimp (Litopenaeus vannamei) cultured at different temperatures [J/OL]. Aquaculture, 2025, 594: 741393 [2025-06-20]. . |
| [16] | HUANG Y, GE R, LOU G, et al.. The influence of dietary coenzyme Q10 on growth performance, antioxidant capacity and resistance against Aeromonas hydrophila of juvenile European eel (Anguilla anguilla) [J/OL]. Fish Shellfish Immunol., 2023, 138: 108834 [2025-06-30]. . |
| [17] | LITTARRU G P, TIANO L. Bioenergetic and antioxidant properties of coenzyme Q10: recent developments [J]. Mol. Biotechnol., 2007, 37(1): 31-37. |
| [18] | BENTINGER M, BRISMAR K, DALLNER G. The antioxidant role of coenzyme Q [J]. Mitochondrion, 2007, 7(Sl): S41-S50. |
| [19] | IGHODARO O M, AKINLOYE O A. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid [J]. Alex. J. Med., 2018, 54(4): 287-293. |
| [20] | BALIOU S, ADAMAKI M, IOANNOU P, et al.. Protective role of taurine against oxidative stress (review) [J/OL]. Mol. Med. Rep., 2021, 24(2): 605 [2025-06-20]. . |
| [21] | SHIN J Y, CHOI J W, KIM D G, et al.. Protective effects of coenzyme Q10 against acute pancreatitis [J/OL]. Int. Immunopharmacol., 2020, 88: 106900 [2025-06-20]. . |
| [22] | TAWFIK M K. Combination of coenzyme Q10 with methotrexate suppresses Freund’s complete adjuvant-induced synovial inflammation with reduced hepatotoxicity in rats: effect on oxidative stress and inflammation [J]. Int. Immunopharmacol., 2015, 24(1): 80-87. |
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