关键词: 马铃薯, GhABF2, 转基因, 抗旱性

Abstract: The transcription factor GhABF2 is homologous to AREB1/ABF2. The drought resistance of plants are improved by overexpressing GhABF2. In order to create the drought-resistant germplasm resources of potato, GhABF2 gene was transferred into potato plantlet of cultivar “Atlantic” and mediated by Agrobacterium tumefaciens and 12 lines of kan-resistant seedlings were obtained. By using PCR、RT-PCR and Southern-blotting, this paper screened out eight transgenic lines with high GhABF2 expression, and investigated their physiological biochemical indexes and growth characteristics under drought conditions. The results showed that the biomass, chlorophyll content, soluble sugar content, proline content,  superoxide dismutase (SOD) and peroxidase (POD) activities of transgenic plants were significantly higher than those of the control under PEG-6000 stress. It was suggested that the drought tolerance of potato was improved by overexpressing GhABF2. The present research provided important genetic resources for drought-tolerant new variety breeding, and had important theoretical and practical significance.

Key words: potato, GhABF2, transgenetic, drought tolerance