关键词: 大豆, 蛋白, 脂肪, QTLs, 上位性效应, 基因互作

Abstract: Soybean is an important crops in the world due to its high seed protein and oil contents, while the contents of seed protein and oil attributed to the quantitative traits which were controlled by multiple minor-genes. Though there were many related QTLs reported, most of them only focused on the single additive QTLs, and few of them payed attentions to the epistatic QTLs. In this paper, a soybean RIL population (ZL) was used to study the epistatic QTLs of seed protein and oil contents by analyzing ZL population using Illumina BARCSoySNP6K Beadchip and detecting the phenotypes under four different environments. The results showed that there were 48 pairs of epistatic QTLs associated with protein content and 55 pairs of epistatic QTLs related to oil content in soybean seeds. Furthermore, there were 19 pairs of co-located epistatic QTLs controlling the protein and oil contents simultaneously. Among these co-located epistatic QTLs, 12 pairs of them shared the same QTL mapping regions, 2 pairs of them shared one same marker and five pairs of them had the QTLs regions not above 5 cM genetic distance, which could be regarded as the same QTL. Meanwhile, the results also showed that these epistatic QTLs located on nineteen chromosomes of soybean except for the chromosome 11. Thus, this study identified many epistatic QTLs for soybean seed protein and oil contents, which not only could be used for soybean quality genetic improvements in future, but also offered some evidences for reveal the negative relationships between seed protein and oil contents in soybean.

Key words: soybean, protein, oil, QTLs, epistatic effects, gene interactions