关键词: 荞麦, 转录组, 从头组装, 差异基因, 黄酮合成基因

Abstract: Fagopyrum gene resources are relatively limited. Taking roots of F. esculentum and F. Cymosum as experimental material, this study conducted transcriptome sequence by Illumina HiSeqTM 2000. After de novo assembly, a total of 64 618 unigenes were obtained, among which 37 546 unigenes were annotated. A total of 4 709 differentially expressed genes (DEGs) was obtained through screening the DEGs between F. esculentum root and F. cymosum root. Among them, 2 460 were upregulated, whereas 2 249 were downregulated. GO annotation of these DEGs were focused on 10 cellular components, 12 molecular function, and 22 biological processes. After upgraded the metabolic pathways, the DEGs involved in 40 and 18 metabolic pathways were significantly enriched in upregulated genes and downregulated genes, respectively. These significantly enriched metabolic pathways were involved in the biological regulatory of F. esculentum root and F. cymosum root. At last, we analyzed the key genes that annotated to the pathway of flavonoid biosynthesis, and a total of 21 genes was obtained. This research not only enriched Fagopyrum gene resources, provided data foundation for molecular biology research of Fagopyrum; but also supplied reference for screening expression tendency of related key genes in F. esculentum root and F. cymosum root.

Key words: Fagopyrum, transcriptome, de novo assembly, differentially expressed genes, flavonoid biosynthesis genes