关键词: 太行菊属, 根际, 微生物, 多样性

Abstract: Opisthopappus  has only two species of Opisthopappus taihangensis (Ling) Shih and Opisthopappus longilobus Shih, which are only distributed in mountainous crevices of high-altitude regions and have a special living environment. In order to analyze the survival mechanism of Opisthopappus plants in special habitat, pH, alkali-N, olsen-P and avail-K contents in rhizosphere soil of O. taihangensis and O. longilobus under wild conditions were detected, and the high-throughput sequencing analysis of rhizosphere microorganisms was carried out. The results showed that the pH of the soil samples was nearly neutral, and the available alkali-N, olsen-P and avail-K contents were low. Miseq sequencing of rhizosphere microorganism showed that Actinobacteria were the dominant bacteria of O. taihangensis and O. longilobus, Ascomycota was their dominant fungi.  The rhizosphere bacterial diversity index of O. taihangensis was higher than that of O. longilobus, while the rhizosphere fungal diversity index of O. taihangensis was lower than that of O. longilobus. The richness of nitrogen cycle, carbon cycle and photosynthetic microorganisms in O. taihangensis were higher than those in O. longilobus, while the richness of stress-resistant microorganisms in O. taihangensis was higher than that in O. longilobus. The diversities of bacteria and fungi in the rhizosphere of O. taihangensis and O. longilobus were significant or extremely significant negative correlated with pH, alkali-N, olsen-P and avail-K of soil. The present research provided an important theoretical basis for the study of ecological adaptation mechanism, scientific protection, development and utilization of Opisthopappus species.

Key words: Opisthopappus sp., rhizosphere, microorganism, diversity