关键词: 肝, 上皮样干细胞, 诱导分化, 北京鸭, 生物学特性

Abstract: In order to study the biological characteristics of liver epithelioid stem cells (LESCs) of Beijing duck, they were isolated, cultured and identified in vitro, and the potential of multidirectional differentiation was tested. The LESCs of Beijing duck were isolated from 17-day-old healthy duck embryos by immunofluorescence, RT-PCR and flow cytometry. The LESCs of Beijing duck were induced differentiation and their differentiation potential were detected. The results showed that LESCs were isolated from duck embryos and successfully differentiated into adipogenic cells and chondroblasts, which proved that LESCs had the potential of multidirectional differentiation of stem cells. Beijing ducks LESCs had strong ability of proliferation, self-renewal and plasticity in vitro. They could be used as germplasm cells to preserve the genetic resources of Beijing duck in the form of stem cells for a long time, and could also provide reference for genetic improvement of Beijing duck.

Key words: liver, epithelioid stem cells, induced differentiation, Beijing duck, biological characteristics