关键词: 小麦叶锈病；TaCIPK31；病毒诱导的基因沉默, 抗性

Abstract: CIPK is a plant-specific serine-threonine protein kinase family belonged to the class of SnRK3 kinase (SNF1-related protein kinase3, SnRK3), which mainly interacts with the calcineurin B-like protein (calcineurin B-like protein) to mediate  plant responses to external stimuli and regulate a wide range of physiological processes. This study obtained a CIPK gene, TaCIPK31, from the wheat leaves infected by Puccinia triticinia (Pt). The full-length cDNA of TaCIPK31 was 1 350 bp, which encoding 449 amino acids. Multi-sequence alignment showed that TaCIPK31 shared high similarity with CIPK in Oryza sativa and Aegilops tauschii, etc. Analysis of the protein domain indicated that TaCIPK31 contained conserved regulatory domain  and protein kinases domain. qRT-PCR assays indicated that TaCIPK31 was induced by Pt infection and significantly expressed during compatible interactions between wheat and Pt. Subcellular localization assays revealed that TaCIPK31 showed similar to that of the GFP control, indicating a cytoplasmic and nuclear localization. Silencing of TaCIPK31 in TcLr37  induced by virus  enhanced resistance of wheat to the virulent Pt isolate THTT. Moreover, detailed histological analysis showed that silencing of TaCIPK31 delayed the colonization of the haustorial mother cell in the host. These results suggested that TaCIPK31 negatively regulated wheat resistance to Pt infection, which provided a reference for revealing the role and molecular mechanism of CIPK protein kinase in wheat-leaf rust interaction system.

Key words: wheat leaf rust disease, TaCIPK31, virus-induced gene silencing, resistance