关键词: 激素, 火焰南天竹, 离体培养

Abstract: Common nandina (Nandina domestica ‘Fire power’.) belongs to Nandina genus, Berberidaceae, and it is the evergreen shrub with important ornamental value in landscape application. Traditional cultivation depends on cottage propagation, but reproduction rate is low, reproductive rate and rooting are still major problems in tissue culture of Nandina domestica ‘Fire power’. In this research, effects of different hormones on adventitious buds induction, multiplication and rooting of Nandina domestica ‘Fire power’ were studied. The results showed that MS+0.3 mg/L 6-BA + 30 g/L sugar was the best induced medium, the induction rate was 93%；the subculture medium for differentiation was MS supplemented added with 1.0 mg/L 6-BA，0.1 mg/L NAA and 30 g/L sugar, the multiplication efficient was 3.5. The optimal rooting medium was 1/2 MS added with 0.3 mg/L IBA and 20 g/L sugar，the rooting rate of plantlets could reach 97%. The seedlings soaked in carbendazim with an effective component content of 50% diluted 1 000 times for 1 h showed high survival rate and good growth. The established propagation system of Nandina domestica ‘Fire power’ had fast propagation rate, and a large number of plants could be obtained in the short term. This propagation technique provided technical support for factory production and meets the market demand.

Key words: hormones, Nandina domestica ‘Fire power’, in vitro culture