关键词: 转基因马铃薯, 标准质粒, 检测, 安全监管

Abstract: According to the global vigorous development momentum of genetically modified (GM) crops, GM potato commercialization has become a general trend. Potato is the 4th important food in the world next to rice, wheat and corn. At present, 45 species of GM potatoes have been approved for commercial cultivation in the world, mainly involved in insect-resistant, virus-resistant and quality improvement, etc. traits. GM technology has brought  huge economic benefits to people, but at the same time raised great concern, including food safety and environment security, etc.. Therefore, the importance of testing and supervising the components of GM product becomes prominent. Within the area of testing GM crops, standard plasmid with the merits of easy obtaining, high purity, low cost, good stability is widely applied. This paper investigated all important information for transgenic potato commercialization, build up multi-target-directed plasmid pBJGMM003 containing anti-potato beetle cry3A gene, nopaline synthase promoter (P-nos) gene, anti-potato virus Y (pvycp) gene and internal control gene patatin (PATA). This plasmid can be used for identification, testing and inspection of GM potatoes. Its detection ability had covered most of GM potatoes. The problem of lacking potato positive standard was solved, thus providing guarantee for the smooth progress of GM potato works.

Key words: genetically modified potato, standard plasmid, detection, safety supervision