关键词: EREB58转录因子, 启动子分析, 顺式作用元件, 间接防御, 玉米

Abstract: It was found that EREB58 transcription factor could regulate the expression of maize terpene synthase gene TPS10 and initiate indirect defense system. But the transcriptional regulation mechanism of EREB58 is still unknown at present. In this study, 1 193 bp promoter sequence of ZmEREB58 was cloned and analyzed by applying bioinformatics. There were several cis-elements in the promoter, which related to plant defense, such as ABRE, Box-W1, GARE motif and TCA elements. The results of GUS histochemical staining and GUS enzyme activity analysis in transgenic Arabidopsis harboring different truncated ZmEREB58 promoter-GUS showed that under normal conditions, EREB58PF1-PF7 and PF9 were not stained, but  PF8 was. After treatment with methyl jasmonate and Asian corn borer, PF1-PF7 were stained, PF8 without significant changes and PF9 still had no GUS staining. The analysis of GUS enzyme activity was consistent with the results of GUS histochemical staining. The above results indicated that the regions from -323 to -270 and -270 to -183 of the ZmEREB58 promoter were key sequences for performing promoter function. The region from -323 to -270 of the ZmEREB58 promoter contained cis-acting element inhibiting ZmEREB58 transcription; in the meantime the region from -270 to -183 of the ZmEREB58 promoter was integral for transcription. These results laid foundation for further studied on the mechanism of EREB58 transcriptional regulation.

Key words: EREB58 transcript factor, promoter analysis, cis-elements, indirect defense, maize