Journal of Agricultural Science and Technology ›› 2024, Vol. 26 ›› Issue (12): 129-137.DOI: 10.13304/j.nykjdb.2023.0589
• ANIMAL AND PLANT HEALTH • Previous Articles Next Articles
Chunyan LI1(), Qian LIANG1(
), Chengxiang GAO1, Zongsuo LIANG2, Fang WANGA1
Received:
2023-08-08
Accepted:
2023-12-18
Online:
2024-12-15
Published:
2024-12-17
Contact:
Qian LIANG
李春燕1(), 梁倩1(
), 高成香1, 梁宗锁2, 王芳1
通讯作者:
梁倩
作者简介:
李春燕E-mail:1602252527@qq.com;
基金资助:
CLC Number:
Chunyan LI, Qian LIANG, Chengxiang GAO, Zongsuo LIANG, Fang WANGA. Study on Insecticidal Activity Constituents and Mechanism of Artemisia annua Against Mythimna separate[J]. Journal of Agricultural Science and Technology, 2024, 26(12): 129-137.
李春燕, 梁倩, 高成香, 梁宗锁, 王芳. 黄花蒿对粘虫杀虫活性成分及机制研究[J]. 中国农业科技导报, 2024, 26(12): 129-137.
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URL: https://nkdb.magtechjournal.com/EN/10.13304/j.nykjdb.2023.0589
馏分 Fraction | 处理时间Treatment time/d | |||
---|---|---|---|---|
1 | 3 | 5 | 7 | |
1 | 4.0±2.5 i | 4.0±2.5 j | 4.0±2.5 n | 4.0±2.5 m |
2 | 32.0±2.0 d | 36.0±2.5 e | 36.0±2.5 d | 36.0±2.5 e |
3 | 32.0±2.0 d | 36.0±2.5 e | 36.0±2.5 d | 36.0±2.5 e |
4 | 4.0±2.5 i | 10.0±0.0 i | 10.0±0.0 l | 10.0±0.0 l |
5 | 18.0±2.0 g | 32.0±3.7 f | 38.0±0.2 d | 56.0±2.5 d |
6 | 28.0±2.0 e | 78.0±2.0 b | 78.0±2.0 b | 78.0±2.0 b |
7 | 46.0±2.5 c | 60.0±0.0 d | 60.0±0.0 c | 70.0±0.0 c |
8 | 100.0±0.0 a | 100.0±0.0 a | 100.0±0.0 a | 100.0±0.0 a |
9 | 66.0±2.5 b | 72.0±2.0 c | 76.0±2.5 b | 76.0±2.5 b |
10 | 6.0±2.5 h | 10.0±0.0 i | 16.0±4.0 j | 20.0±5.5 h |
11 | 4.0±2.5 i | 10.0±0.0 i | 12.0±2.0 k | 14.0±2.5 j |
12 | 12.0±2.0 g | 20.0±0.0 g | 20.0±0.0 i | 24.0±2.5 g |
13 | 2.0±2.0 i | 10.0±0.0 i | 12.0±2.0 k | 12.0±2.0 k |
14 | 34.0±2.5 d | 42.0±2.0 e | 42.0±2.0 d | 42.0±2.0 e |
15 | 34.0±2.5 d | 36.0±2.5 e | 38.0±2.0 d | 40.0±0.0 e |
16 | 2.0±2.0 i | 8.0±2.0 j | 8.0±2.0 m | 8.0±2.0 l |
17 | 12.0±2.0 g | 18.0±2.0 g | 20.0±0.0 i | 20.0±0.0 h |
18 | 12.0±2.0 g | 16.0±2.5 h | 18.0±2.0 i | 18.0±2.0 i |
19 | 28.0±2.0 e | 32.0±2.0 f | 36.0±2.5 d | 36.0±2.5 e |
20 | 16.0±2.5 g | 18.0±2.0 g | 18.0±2.0 i | 18.0±2.0 i |
21 | 4.0±2.5 i | 6.0±2.5 j | 8.0±2.0 m | 8.0±2.0 l |
22 | 36.0±2.5 d | 40.0±0.0 e | 40.0±0.0 d | 40.0±0.0 e |
23 | 14.0±2.5 g | 22.0±2.0 g | 26.0±2.5 g | 26.0±2.5 g |
24 | 18.0±2.0 g | 22.0±2.0 g | 24.0±2.5 h | 24.0±2.5 g |
25 | 18.0±2.0 g | 24.0±2.5 g | 30.0±0.0 f | 30.0±0.0 f |
26 | 26.0±4.0 f | 32.0±2.0 f | 34.0±2.5 e | 38.0±2.0 e |
27 | 6.0±2.5 h | 6.0±2.5 j | 10.0±0.0 | 10.0±0.0 l |
Table 1 Corrected mortality of 27 fractons from A. annua against M. separatain
馏分 Fraction | 处理时间Treatment time/d | |||
---|---|---|---|---|
1 | 3 | 5 | 7 | |
1 | 4.0±2.5 i | 4.0±2.5 j | 4.0±2.5 n | 4.0±2.5 m |
2 | 32.0±2.0 d | 36.0±2.5 e | 36.0±2.5 d | 36.0±2.5 e |
3 | 32.0±2.0 d | 36.0±2.5 e | 36.0±2.5 d | 36.0±2.5 e |
4 | 4.0±2.5 i | 10.0±0.0 i | 10.0±0.0 l | 10.0±0.0 l |
5 | 18.0±2.0 g | 32.0±3.7 f | 38.0±0.2 d | 56.0±2.5 d |
6 | 28.0±2.0 e | 78.0±2.0 b | 78.0±2.0 b | 78.0±2.0 b |
7 | 46.0±2.5 c | 60.0±0.0 d | 60.0±0.0 c | 70.0±0.0 c |
8 | 100.0±0.0 a | 100.0±0.0 a | 100.0±0.0 a | 100.0±0.0 a |
9 | 66.0±2.5 b | 72.0±2.0 c | 76.0±2.5 b | 76.0±2.5 b |
10 | 6.0±2.5 h | 10.0±0.0 i | 16.0±4.0 j | 20.0±5.5 h |
11 | 4.0±2.5 i | 10.0±0.0 i | 12.0±2.0 k | 14.0±2.5 j |
12 | 12.0±2.0 g | 20.0±0.0 g | 20.0±0.0 i | 24.0±2.5 g |
13 | 2.0±2.0 i | 10.0±0.0 i | 12.0±2.0 k | 12.0±2.0 k |
14 | 34.0±2.5 d | 42.0±2.0 e | 42.0±2.0 d | 42.0±2.0 e |
15 | 34.0±2.5 d | 36.0±2.5 e | 38.0±2.0 d | 40.0±0.0 e |
16 | 2.0±2.0 i | 8.0±2.0 j | 8.0±2.0 m | 8.0±2.0 l |
17 | 12.0±2.0 g | 18.0±2.0 g | 20.0±0.0 i | 20.0±0.0 h |
18 | 12.0±2.0 g | 16.0±2.5 h | 18.0±2.0 i | 18.0±2.0 i |
19 | 28.0±2.0 e | 32.0±2.0 f | 36.0±2.5 d | 36.0±2.5 e |
20 | 16.0±2.5 g | 18.0±2.0 g | 18.0±2.0 i | 18.0±2.0 i |
21 | 4.0±2.5 i | 6.0±2.5 j | 8.0±2.0 m | 8.0±2.0 l |
22 | 36.0±2.5 d | 40.0±0.0 e | 40.0±0.0 d | 40.0±0.0 e |
23 | 14.0±2.5 g | 22.0±2.0 g | 26.0±2.5 g | 26.0±2.5 g |
24 | 18.0±2.0 g | 22.0±2.0 g | 24.0±2.5 h | 24.0±2.5 g |
25 | 18.0±2.0 g | 24.0±2.5 g | 30.0±0.0 f | 30.0±0.0 f |
26 | 26.0±4.0 f | 32.0±2.0 f | 34.0±2.5 e | 38.0±2.0 e |
27 | 6.0±2.5 h | 6.0±2.5 j | 10.0±0.0 | 10.0±0.0 l |
序号 No. | 保留时间 Retention time/min | 化合物 Compound | 相对含量 Relative content/% |
---|---|---|---|
1 | 8.7 | 十三烷Tridecane | 7.9 |
2 | 10.3 | 丁香酚Eugenol | 1.4 |
3 | 15.3 | 4-喹啉甲醛 4-quinolinecarboxaldehyde | 72.8 |
4 | 16.6 | 石竹烯氧化物Caryophyllene oxide | 3.6 |
5 | 17.9 | 9,10-脱氢环异龙胆烯 9,10-dehydro-cycloisolongifolene | 1.9 |
6 | 23.4 | 6,10,14-三甲基-2-十五烷酮 6,10,14-trimethyl-2-pentadecanone | 1.0 |
7 | 30.9 | 亚油酸乙酯 Linoleic acid ethyl ester | 2.4 |
8 | 31.0 | 亚麻酸乙酯 Linolenic acid ethyl ester | 3.1 |
Table 2 Chemical composition of fraction 8
序号 No. | 保留时间 Retention time/min | 化合物 Compound | 相对含量 Relative content/% |
---|---|---|---|
1 | 8.7 | 十三烷Tridecane | 7.9 |
2 | 10.3 | 丁香酚Eugenol | 1.4 |
3 | 15.3 | 4-喹啉甲醛 4-quinolinecarboxaldehyde | 72.8 |
4 | 16.6 | 石竹烯氧化物Caryophyllene oxide | 3.6 |
5 | 17.9 | 9,10-脱氢环异龙胆烯 9,10-dehydro-cycloisolongifolene | 1.9 |
6 | 23.4 | 6,10,14-三甲基-2-十五烷酮 6,10,14-trimethyl-2-pentadecanone | 1.0 |
7 | 30.9 | 亚油酸乙酯 Linoleic acid ethyl ester | 2.4 |
8 | 31.0 | 亚麻酸乙酯 Linolenic acid ethyl ester | 3.1 |
处理时间/d Treatment time | 化合物 Compound | 半数致死浓度 LC50/(mg∙mL-1) | 95%置信区间 95% CI | 斜率 Slope | 卡方 χ2 | P值 P value |
---|---|---|---|---|---|---|
1 | 十三烷Tridecane | 10.0 d | 8.6~15.6 | 1.8±0.2 | 3.6 | 0.3 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 9.8 c | 8.1~12.9 | 2.2±0.2 | 4.1 | 0.3 | |
石竹烯氧化物 Caryophyllene oxide | 34.6 e | 19.4~108.6 | 1.4±0.3 | 2.6 | 0.5 | |
亚油酸乙酯 Linoleic acid ethyl ester | 6.6 b | 4.9~10.1 | 3.5±0.3 | 8.4 | 0.0 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 4.6 a | 2.4~16.6 | 2.2±0.2 | 22.1 | 0.0 | |
3 | 十三烷 Tridecane | 9.8 d | 7.5~14.2 | 1.5±0.2 | 0.3 | 1.0 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 5.8 b | 4.8~7.4 | 1.7±0.2 | 1.4 | 0.7 | |
石竹烯氧化物 Caryophyllene oxide | 33.6 e | 18.0~113.4 | 1.1±0.2 | 0.5 | 0.9 | |
亚油酸乙酯 Linoleic acid ethyl ester | 6.4 c | 4.4~12.2 | 2.5±0.2 | 9.4 | 0.0 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 3.1 a | 1.6~7.5 | 1.8±0.2 | 15.8 | 0.0 | |
5 | 十三烷 Tridecane | 9.3 e | 7.1~13.5 | 1.4±0.2 | 1.1 | 0.8 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 2.6 b | 0.2~79.2 | 1.1±0.1 | 15.2 | 0.0 | |
石竹烯氧化物 Caryophyllene oxide | 8.8 d | 4.6~61.3 | 0.5±0.1 | 0.7 | 0.9 | |
亚油酸乙酯 Linoleic acid ethyl ester | 5.5 c | 3.6~10.6 | 2.1±0.2 | 9.0 | 0.0 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 2.0 a | 1.2~3.2 | 1.7±0.2 | 8.1 | 0.0 | |
7 | 十三烷 Tridecane | 7.9 e | 6.2~10.7 | 1.5±0.2 | 2.3 | 0.5 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 2.5 b | 0.0~9 134 638.4 | 1.1±0.1 | 17.8 | 0.0 | |
石竹烯氧化物 Caryophyllene oxide | 3.6 c | 1.8~13.0 | 0.4±0.1 | 0.31 | 1.0 | |
亚油酸乙酯 Linoleic acid ethyl ester | 5.4 d | 3.7~9.6 | 2.1±0.2 | 7.2 | 0.1 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 1.5 a | 1.2~1.8 | 1.6±0.2 | 4.6 | 0.2 |
Table 3 Contact toxicity of five compounds against M. separatain
处理时间/d Treatment time | 化合物 Compound | 半数致死浓度 LC50/(mg∙mL-1) | 95%置信区间 95% CI | 斜率 Slope | 卡方 χ2 | P值 P value |
---|---|---|---|---|---|---|
1 | 十三烷Tridecane | 10.0 d | 8.6~15.6 | 1.8±0.2 | 3.6 | 0.3 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 9.8 c | 8.1~12.9 | 2.2±0.2 | 4.1 | 0.3 | |
石竹烯氧化物 Caryophyllene oxide | 34.6 e | 19.4~108.6 | 1.4±0.3 | 2.6 | 0.5 | |
亚油酸乙酯 Linoleic acid ethyl ester | 6.6 b | 4.9~10.1 | 3.5±0.3 | 8.4 | 0.0 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 4.6 a | 2.4~16.6 | 2.2±0.2 | 22.1 | 0.0 | |
3 | 十三烷 Tridecane | 9.8 d | 7.5~14.2 | 1.5±0.2 | 0.3 | 1.0 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 5.8 b | 4.8~7.4 | 1.7±0.2 | 1.4 | 0.7 | |
石竹烯氧化物 Caryophyllene oxide | 33.6 e | 18.0~113.4 | 1.1±0.2 | 0.5 | 0.9 | |
亚油酸乙酯 Linoleic acid ethyl ester | 6.4 c | 4.4~12.2 | 2.5±0.2 | 9.4 | 0.0 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 3.1 a | 1.6~7.5 | 1.8±0.2 | 15.8 | 0.0 | |
5 | 十三烷 Tridecane | 9.3 e | 7.1~13.5 | 1.4±0.2 | 1.1 | 0.8 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 2.6 b | 0.2~79.2 | 1.1±0.1 | 15.2 | 0.0 | |
石竹烯氧化物 Caryophyllene oxide | 8.8 d | 4.6~61.3 | 0.5±0.1 | 0.7 | 0.9 | |
亚油酸乙酯 Linoleic acid ethyl ester | 5.5 c | 3.6~10.6 | 2.1±0.2 | 9.0 | 0.0 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 2.0 a | 1.2~3.2 | 1.7±0.2 | 8.1 | 0.0 | |
7 | 十三烷 Tridecane | 7.9 e | 6.2~10.7 | 1.5±0.2 | 2.3 | 0.5 |
4-喹啉甲醛 4-quinolinecarboxaldehyde | 2.5 b | 0.0~9 134 638.4 | 1.1±0.1 | 17.8 | 0.0 | |
石竹烯氧化物 Caryophyllene oxide | 3.6 c | 1.8~13.0 | 0.4±0.1 | 0.31 | 1.0 | |
亚油酸乙酯 Linoleic acid ethyl ester | 5.4 d | 3.7~9.6 | 2.1±0.2 | 7.2 | 0.1 | |
亚麻酸乙酯 Linolenic acid ethyl ester | 1.5 a | 1.2~1.8 | 1.6±0.2 | 4.6 | 0.2 |
Fig. 2 Activity of target enzymes and metabolic enzymes against M. separatain under different treatmentsNote:Different Greece letters indicate significant differences between different treatment groups of same treatment time at P<0.05 level; different English letters indicate significant differences between different treatment times of same treatment at P<0.05 level.
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