Abstract: To explore the influence of different biochar dosages on the carbon metabolism of rhizospheric soil microorganism community of flue-cured tobacco, and clarify the appropriate biochar dosage, this study took Yunyan 87 as experimental material and established a contrast group(CK) with no application of biochar and 3 treatments with biochar doses as 3 000 kg/hm2（T1）, 3 750 kg/hm2（T2）, and 4 500 kg/hm2（T3）, respectively. The soil samples were collected during the fast growing period of flue-cured tobacco, and the functional diversity of rhizospheric soil microorganism community of flue-cured tobacco were analyzed by Biolog-ECO microplate technology. The results showed that: The biochar application could increase the AWCD value and various biodiversity indexes of soil microorganism, and significantly increase the utilization of carboxylic acids and polymer carbon source. T2 utilization of these 2 carbon resource categories were increased than that of the CK by 24.82% and 70.71%, respectively. The analysis of principal components indicated that carbohydrates, carboxylic acids and amino acids were the relevant carbon resources causing more differences in carbon metabolism features of soil microorganism. In conclusion, T2 showed the best capability in utilizing carbon resources, and the optimum biochar dosage was 3 750 kg/hm2.

Key words: biochar, flue-cured tobacco, microbial, carbon metabolism, Biolog-ECO

摘要： 为探究不同施用量的生物炭对烤烟根际土壤微生物群落碳代谢的影响，并明确适宜的生物炭用量。以云烟87为试验材料，设置了不施用生物炭的对照组（CK）和生物炭用量分别为3 000 kg/hm2（T1）、3 750 kg/hm2（T2）与4 500 kg/hm2（T3）的处理组，于烤烟旺长期采集土壤样品，运用Biolog-ECO微孔板技术分析烤烟根际土壤微生物群落的功能多样性。结果表明：施用生物炭能提高土壤微生物AWCD值与微生物多样性各项指数，能显著提高羧酸类和聚合物类碳源的利用能力，且T2处理在这两类碳源利用能力上较CK处理分别显著提高24.82%和70.71%。主成分分析表明，碳水化合物类、羧酸类和氨基酸类碳源是造成各处理土壤微生物碳代谢特征存在较大差异的相关碳源。综合来看，T2处理土壤微生物对碳源的利用能力最强，最适宜的生物炭施用量为3 750 kg/hm2。

关键词: 生物炭, 烤烟, 微生物, 碳代谢, Biolog-ECO