Abstract: In order to study the poplar diversity and differences and connection among different molecular marker technology, this paper analyzed DNA polmorphism of 12 poplar samples by SSR and ITS markers, and conducted correlation analysis of these 2 markers on the basis of genetic distance. SSR marker indicated that 54 fragments were amplified by 13 pairs primers and polymorphic bands were 51. The PIC (polymorphism information content) value of these markers was varied from 0.36~0.76, and could preferably distinguish different samples. The results of ITS analysis showed that the differences between tested poplar not only expressed in basic group, but also in sequence length.  The results of all samples clustering analysis by these 2 marker methods showed that the two were consistent in faction classification.But there were differences between SSR  and ITS markers in subtle classification, SSR marker could more accurately identify the plants with same source or similar individual plants than ITS marker. The correlation analysis of these 2 methods indicated that they were significantly correlated, the correlation coefficient R reached 0.593 (P=0.01, n=66, rα=0.314 966). Thus, SSR and ITS markers could all be used for poplar variety identification. Combining with the genetic differences of these 2 molecular markers, this paper provided technical basis for the rapid and accurate identification of poplars germplasm resources.

Key words: Populus, SSR, ITS, cluster analysis

摘要： 为研究杨树多样性和不同分子标记技术间的差异与联系，采用SSR和ITS标记分析12个杨树样品的DNA多态性，并在遗传距离的基础上对这两种标记方法进行相关性分析。SSR标记表明，13对引物共扩增条带54条，多态性条带51条，PIC值为0.36～0.76，能较好的区分不同样品。ITS分析结果显示，供试杨树之间差异不仅表现在碱基的差异，也表现在序列长度的差异。利用这两种标记方法对所有样品进行聚类分析发现，二者在派别间分类基本一致，但在细微分类中SSR标记相对于ITS技术能更准确的鉴别出来源相同或相似的植株个体。两种标记方法相关性分析表明，二者显著相关，相关系数R达到0.593（P=0.01，n=66，rα=0314 966）。由此可见，它们均可用作杨树品种鉴定，同时结合这两种分子标记的遗传差异，可为快速准确的鉴定杨树资源提供技术依据。

关键词: 杨树, SSR, ITS, 聚类分析