Abstract: In order to  promot seed germination of Buplerurum chinense DC in field cultivation, the seeds were used as materials and soaked in mixed liquor with different ratio of CS and MeJA for 24 h. Seeds soaked in pure water were taken as the CK. Then moisture content, volum, germination rate, soluble sugar and soluble protein contents, and free amino acids contents of the seeds were measured and analyzed. The results showed that all MeJA and CS mixed liquor could promote seed germination rate. At 35 d,  the germination rates of all treatments were significantly higher than that of the CK. The germination rates of all treatments were higher than the CK significantly. But there was no significant difference between T3 and T4, and there was significant difference between them and the other treatments. The relative maximum change rate of moisture content of T2 was the biggest, and that of T3 and the CK were the least. Except T3 and the CK, there were significant differences among the other treatments. The volume of all treatments showed first down and then up trend. T4 had the biggest volume. The total soluble sugar content showed down-up-down-up wave change trend. During seed germination process, the overall soluble protein contents showed decreasing tendency. After 35 d, the amino acid contents of the CK and T3 were higher than before, while that of T1, T2 and T4 were lower than the initial stage. Comprehensive evaluation by subordination function analysis showed that soaking seed with the mixture of 10 mg/mL CS and 224.3 μg/mL MeJA had the best effect. The germination rate could reach 54%. These results provided reference for promoting seed germination in aritificial cultivation of Bupleum chinense.

Key words: Bupleurum chinense DC., chitosan(CS), methyl jasmonate(MeJA), seed germination

摘要： 为促进大田栽培过程中北柴胡种子的萌发率,以北柴胡(Buplerurum chinense DC.)种子为试验材料，用不同配比的茉莉酸甲酯、壳聚糖混合液进行浸种24 h；以清水浸种作为CK，测定分析种子含水量、体积、发芽率及可溶性糖、可溶性蛋白、游离氨基酸含量等指标。结果表明：不同混合液浸种均有促进北柴胡种子萌发的作用。在发芽率最高的35 d，混合液处理后发芽率均显著高于对照，但T3和T4 处理差异不显著，T3和T4与其他处理间差异显著；T2处理的种子含水量的最大相对变化率最大，T3和对照差异最小，除T3和对照外，其他处理间差异显著；体积呈现先下降再上升的趋势，且T4体积最大；可溶性糖含量整体出现下降-上升-下降-上升的波状变化；在种子萌发过程中可溶性蛋白质含量整体呈现减少趋势；到35 d时，CK和T3氨基酸含量比初始含量是增加的，而T1、T2和T4氨基酸含量比初始含量低。通过隶属函数分析进行综合评价，筛选出壳聚糖10 mg/mL与茉莉酸甲酯224.3 μg/mL组合浸种效果最好，发芽率为54%；为北柴胡人工栽培过程中促进种子萌发效率提供参考。

关键词: 北柴胡, 壳聚糖, 茉莉酸甲酯, 种子萌发