Abstract:

The AP2 transcription factor subfamily widely exists in plants, and plays a very important role in regulating plant development. The previous studies showed that SUPERNUMERARY BRACT (OsSNB) was a member of AP2 transcription factor subfamily containing 2 conservative AP2 domains. And OsSNB was involved in regulating the transition from spikelet meristem to floral meristem and the development of floral organ. However, it is not clear whether OsSNB is involved in abiotic stress responses. In this study,  OsSNB gene was obtained from the RAP-DB rice database. In order to investigate the role of OsSNB in abiotic stress and hormone response, sequence analysis revealed that OsSNB promoter contained multiple hormone and stress responsive cis-acting elements, including GCC-box, ABRE, DRE and WRKY. Gene express analysis showed that OsSNB gene could be duressed by abiotic stress salt, drought and induced by hormones ABA and ethylene precursor ACC. These results suggested that OsSNB gene might be involved in regulating the expression of genes responsive to stresses, implying that OsSNB might have important functions in plant growth, development, and abiotic stress responses.

Key words: abiotic stress, hormones, AP2 transcription factors, gene expression

摘要：

AP2转录因子家族普遍存在于植物中，在调控植物发育过程中起到非常重要的作用。前期研究表明，SUPERNUMERARY BRACT(OsSNB)属于AP2转录因子亚家族成员，含有两个保守的AP2结构域，主要参与调控小穗分生组织向花分生组织的转换以及花器官的发育。利用RAP-DB水稻数据库搜索到基因OsSNB,通过序列分析发现 OsSNB启动子序列中含有GCC-box、ABRE、DRE、WRKY等应答非生物胁迫及激素信号的元件；基因表达分析表明，OsSNB基因的表达受NaCl、干旱胁迫和激素ABA以及乙烯前体ACC的诱导。这些结果表明水稻OsSNB基因可能参与调控逆境胁迫反应，在植物生长发育和非生物胁迫的应答中均具有重要功能。

关键词: 非生物胁迫；激素；AP2转录因子；基因表达