Abstract:

In order to investigate the drug resistance of Escherichia coli isolated from pig manuer of large\|scale pig farm and local farmers piggeries on commonly used antimicrobial drugs in Baijiantan region of Karamay City, Xinjiang, this experiment conducted test with minimal inhibitory concentrations (MIC) of antimicrobial drugs on these isolates by broth micro\|dilution method. The results showed that 701 Escherichia coli were isolated from 703 fecal samples (including 549 isolates from large\|scale pig farm and 152 isolates from farmers piggeries). The isolated Escherichia coli were highly resistant to ampicillin and amoxicillin / clavulanic by over 77.0%. These isolates were very sensitive to amikacin. Their tolerant rate reached 11.1%. Isolates tolerant to 3 or more than 3 antimicrobial drugs accounted for 17.0%. In this region, the tolerance of pig Escherichia coli to commonly used antimicrobial drugs was very serious, so in clinical treatment of bacterial diseases, the use of insensitive antimicrobial drugs should be avoid. The tolerant rate of Escherichia coli isolated from large\|scale pig farm was lower than that from the farmers piggeries.

Key words: Escherichia coli from pigs, antimicrobial drug, tolerance, minimum inhibitory concentration

摘要：

为调查新疆克拉玛依市白碱滩地区规模化猪场和养殖户家猪粪样中分离的大肠杆菌对临床常用抗菌药物的耐药情况。对该地区猪场及周边养殖户家猪粪样分离的大肠杆菌采用微量肉汤稀释法进行最小抑菌浓度测定。结果显示：采集的703份粪样中分离出大肠杆菌701株（包括规模化养殖场549株和养殖户152株），分离的大肠杆菌对氨苄西林和阿莫西林/克拉维酸耐药率最高，达77.0%以上；对阿米卡星最敏感，耐药率也达11.1%；3耐以上的菌株占17.0%。该地区猪源大肠杆菌对常用抗菌药物耐药情况较为严重，须在临床治疗细菌性疾病中避开使用不敏感的抗菌药物。规模化养殖场分离的猪源大肠杆菌相比养殖户分离的猪源大肠杆菌对被检抗菌药物的耐药率低。

关键词: 猪源大肠杆菌；抗菌药物；耐药；最小抑菌浓度