Establishment of Comprehensive Evaluation and Prediction Equation for Cucumber Wet-cold Tolerance at Seedling Stage

doi:10.13304/j.nykjdb.2021.0484

Abstract

Abstract:

To establish the evaluation system of cucumber on wet-cold tolerance， the high humidity and low temperature environment was set in growth chambers. The morphological and physiological indexes of 20 cucumber varieties at seedling stage were determined for evaluating the wet-cold tolerance. And the prediction equation of cucumber wet-cold tolerance was established by principal component， membership function and stepwise regression analysis. The results showed that the prediction equation of cucumber wet-cold tolerance was established： Y=0.492+0.032 I₆-0.084 I₄-0.029 I₇， in which， Y represented the comprehensive value， I₄， I₆ and I₇ represented the relative values of catalase activity， proline content and polyphenol oxidase activity in the high humidity and low temperature environment and normal environment， respectively. The higher the Y value was， the more tolerant to wet-cold the cucumber would be. Validation analysis showed that the average accuracy of the prediction equation was 95.9%. It showed that the prediction equation could be used to evaluate wet-cold tolerance of cucumber， which provided technique supports for wet-cold tolerance breeding and cultivation of cucumber.

Key words: cucumber, wet-cold tolerance, identification index, prediction equation

摘要：

为建立黄瓜耐湿冷鉴定评价体系，利用人工气候箱设置湿冷环境处理，通过主成分析和隶属函数分析筛选黄瓜幼苗期耐湿冷性的形态和生理指标，通过逐步回归建立耐湿冷性预测方程。结果表明，黄瓜苗期耐湿冷性方程为：Y=0.492+0.032 I₆-0.084 I₄-0.029 I₇，其中，Y代表综合耐湿冷能力，其值越高表明该品种的耐湿冷性越强；I₄、I₆和I₇分别代表相对过氧化氢酶活性、相对脯氨酸含量和相对多酚氧化酶活性。验证分析表明，该预测方程的平均精确度为95.9%，可用于黄瓜耐湿冷性评价，为黄瓜耐湿冷育种和设施栽培提供了技术支持。

关键词: 黄瓜, 耐湿冷性, 鉴定指标, 预测方程

CLC Number:

S642.2

Yuanyuan DING, Xi’ao WANG, Ce LIU, Shuju LI, Zhihui CHENG. Establishment of Comprehensive Evaluation and Prediction Equation for Cucumber Wet-cold Tolerance at Seedling Stage[J]. Journal of Agricultural Science and Technology, 2022, 24(11): 87-96.

丁圆圆, 王曦奥, 刘策, 李淑菊, 程智慧. 黄瓜耐湿冷性苗期综合评价预测方程的建立[J]. 中国农业科技导报, 2022, 24(11): 87-96.

Figures/Tables 9

Table 1 Information of test materials

缩写

Abbreviation

品种

Variety

来源

Source

耐湿冷性

Wet-cold tolerance

Zn26

中农26

Zhongnong 26

中国农业科学院蔬菜花卉研究所

Institute of Vegetable and Flower， Chinese Academy of Agricultural Sceinces

中 Moderate

Syz

三叶早

Sanyezao

泰安禾元有限公司

Taian Heyuan Co.， Ltd.

敏感 Sensitive

Jyys

京研优胜

Jingyanyousheng

国家蔬菜工程中心

National Vegetable Engineering Center

中 Moderate

QsF1

秋胜F1

Qiusheng F1

吉林环球有限公司

Jilin Huanqiu Co.， Ltd.

弱 Weak

Ymt2188

雅美特2188

Yameite 2188

寿光欣欣然有限公司

Shouguang Xinxinran Co.， Ltd.

弱 Weak

FyF1

富阳F1

Fuyang F1

寿光洪亮有限公司

Shouguang Hongliang Co.， Ltd.

弱 Weak

Jy41*

津优41

Jinyou 41

天津科润黄瓜所

Tianjin Kerun Cucumber Institute

敏感 Sensitive

Table 1 Information of test materials

缩写

Abbreviation

品种

Variety

来源

Source

耐湿冷性

Wet-cold tolerance

Jy4*

津优4号

Jinyou 4

天津科润黄瓜所

Tianjin Kerun Cucumber Institute

中 Moderate

Zn28*

中农28

Zhongnong 28

中科院蔬菜花卉研究所

Institute of Vegetable and Flower， Chinese Academy of Agricultural Sceinces

强 High

Hl35

荷兰35

Helan 35

寿光宏伟种业有限公司

Shouguang Hongwei Co.， Ltd.

弱 Weak

Sy4

疏研4号

Shuyan 4

湖南农科所

Hunan Academy of Agricultural Sciences

敏感 Sensitive

Jy1

津优1号

Jinyou 1

天津科润黄瓜研究所

Tianjin Kerun Cucumber Institute

强 High

Jy64

津优64号

Jinyou 64

天津科润黄瓜研究所

Tianjin Kerun Cucumber Institute

强 High

Bn5

博耐5号

Bonai 5

天津德瑞特种业有限公司

Tianjin Derit Co.， Ltd.

强 High

Msjp313

妙收金品313

Miaoshoujinpin 313

北京妙收有限公司

Beijing Miaoshou Co.， Ltd.

敏感 Sensitive

Ccmc

长春密刺

Changchunmici

沈阳谷雨有限公司

Shenyang Guyu Co.， Ltd.

中 Moderate

Jy35*

津优35

Jinyou 35

天津科润黄瓜研究所

Tianjin Kerun Cucumber Institute

弱 Weak

Rg2*

瑞光2号

Ruiguang 2

国家蔬菜工程中心

National Vegetable Engineering Center

弱 Weak

Sy11*

疏研11号

Shuyan 11

湖南农科所

Hunan Academy of Agricultural Sciences

敏感 Sensitive

Drt8-9F*

德瑞特8-9F

Deruite 8-9F

天津德瑞特有限公司

Tianjin Derit Co.， Ltd.

中 Moderate

Table 2 Relative values of different indexes under wet?cold treatment of different cucumber varieties

品种 Variety	过氧化氢酶 CAT	苯丙氨酸解氨酶 PAL	多酚氧化酶PPO	超氧阴离子 $O 2 -$	谷胱甘肽 GSH	可溶性糖 SS	脯氨酸 Pro	株高 SH	茎粗 SD	叶长 LL
Zn26	0.93±0.012 d	1.35±0.095 cdef	1.01±0.053 gh	1.73±0.107 bcd	1.58±0.077 c	1.32±0.062 ab	3.94±0.078 c	0.67±0.029 abc	0.89±0.078 abc	0.98±0.008 a
Syz	0.62±0.088 e	0.96±0.021 g	0.70±0.057 i	1.25±0.098 gh	1.48±0.098 c	0.61±0.049 g	1.80±0.074 d	0.59±0.017 bc	0.82±0.016 bc	0.71±0.037 c
FyF1	1.22±0.061 c	1.00±0.033 g	1.372±0.029 ef	2.17±0.065 a	1.12±0.056 d	0.90±0.042 ef	3.73±0.402 c	0.64±0.024 abc	0.92±0.029 ab	0.90±0.024 ab
Sy4	1.28±0.120 c	1.48±0.119 bcd	2.50±0.045 b	1.99±0.082 ab	1.42±0.081 c	1.24±0.087 bc	1.13±0.087 e	0.59±0.008 bc	0.88±0.024 abc	0.89±0.017 ab
Jy64	2.17±0.049 a	1.38±0.115 cde	1.47±0.041 de	1.04±0.133 hi	2.58±0.082 a	0.85±0.021 efg	5.45±0.093 a	0.73±0.024 ab	0.96±0.024 a	0.83±0.029 bc
Bn5	0.58±0.061 e	1.05±0.107 fg	1.52±0.029 de	1.26±0.075 fgh	1.54±0.074 c	1.54±0.066 a	5.37±0.107 a	0.78±0.024 a	0.90±0.037 abc	0.90±0.024 ab
Hl35	1.11±0.049 cd	1.70±0.119 b	3.44±0.172 a	1.53±0.050 def	0.33±0.021 f	0.98±0.049 cde	3.47±0.021 bc	0.61±0.033 bc	0.81±0.016 bc	0.89±0.005 ab
Ccmc	1.07±0.074 cd	2.59±0.049 a	2.06±0.078 c	1.45±0.054 efg	0.76±0.025 e	0.85±0.021 efg	3.77±0.021 bc	0.71±0.016 ab	0.94±0.029 ab	0.98±0.021 a
Jy1	0.59±0.039 e	1.05±0.033 g	1.66d±0.057 d	1.03±0.021 hi	2.12±0.024 b	1.31±0.071 ab	4.61±0.062 b	0.74±0.033 ab	0.87±0.012 abc	0.89±0.012 ab
Ymt2188	1.11±0.080 cd	1.09±0.020 efg	0.56±0.070 i	1.82±0.017 bc	1.01±0.050 d	1.27±0.155 b	1.22±0.049 e	0.52±0.026 c	0.80±0.088 c	0.85±0.057 b
QsF1	1.57±0.078 b	1.08±0.045 efg	1.11±0.012 fg	1.66±0.086 cde	1.17±0.046 d	0.67±0.087 fg	3.68±0.050 bc	0.67±0.045 ab	0.85±0.034 abc	0.82±0.057 b
Jyys	0.96±0.069 d	1.51±0.091 bc	1.20±0.066 fg	1.25±0.033 fgh	1.24±0.008 d	0.90±0.021 def	3.36±0.109 c	0.70±0.057 ab	0.89±0.033 abc	0.85±0.005 b
Msjp313	1.59±0.091 b	1.21±0.086 defg	0.80±0.029 hi	1.86±0.036 bc	1.03±0.033 d	1.17±0.070 bcd	1.05±0.037 e	0.60±0.049 bc	0.87±0.037 abc	0.86±0.037 b
均值Mean	1.13	1.34	1.50	1.55	1.35	1.04	3.25	0.65	0.87	0.87
标准差SD	0.43	0.44	0.78	0.38	0.58	0.28	1.49	0.08	0.06	0.07
变异系数CV/%	38.05	32.84	52.00	24.52	42.96	26.92	45.85	12.31	6.90	8.04

Table 2 Relative values of different indexes under wet?cold treatment of different cucumber varieties

品种 Variety	过氧化氢酶 CAT	苯丙氨酸解氨酶 PAL	多酚氧化酶PPO	超氧阴离子 $O 2 -$	谷胱甘肽 GSH	可溶性糖 SS	脯氨酸 Pro	株高 SH	茎粗 SD	叶长 LL
Zn26	0.93±0.012 d	1.35±0.095 cdef	1.01±0.053 gh	1.73±0.107 bcd	1.58±0.077 c	1.32±0.062 ab	3.94±0.078 c	0.67±0.029 abc	0.89±0.078 abc	0.98±0.008 a
Syz	0.62±0.088 e	0.96±0.021 g	0.70±0.057 i	1.25±0.098 gh	1.48±0.098 c	0.61±0.049 g	1.80±0.074 d	0.59±0.017 bc	0.82±0.016 bc	0.71±0.037 c
FyF1	1.22±0.061 c	1.00±0.033 g	1.372±0.029 ef	2.17±0.065 a	1.12±0.056 d	0.90±0.042 ef	3.73±0.402 c	0.64±0.024 abc	0.92±0.029 ab	0.90±0.024 ab
Sy4	1.28±0.120 c	1.48±0.119 bcd	2.50±0.045 b	1.99±0.082 ab	1.42±0.081 c	1.24±0.087 bc	1.13±0.087 e	0.59±0.008 bc	0.88±0.024 abc	0.89±0.017 ab
Jy64	2.17±0.049 a	1.38±0.115 cde	1.47±0.041 de	1.04±0.133 hi	2.58±0.082 a	0.85±0.021 efg	5.45±0.093 a	0.73±0.024 ab	0.96±0.024 a	0.83±0.029 bc
Bn5	0.58±0.061 e	1.05±0.107 fg	1.52±0.029 de	1.26±0.075 fgh	1.54±0.074 c	1.54±0.066 a	5.37±0.107 a	0.78±0.024 a	0.90±0.037 abc	0.90±0.024 ab
Hl35	1.11±0.049 cd	1.70±0.119 b	3.44±0.172 a	1.53±0.050 def	0.33±0.021 f	0.98±0.049 cde	3.47±0.021 bc	0.61±0.033 bc	0.81±0.016 bc	0.89±0.005 ab
Ccmc	1.07±0.074 cd	2.59±0.049 a	2.06±0.078 c	1.45±0.054 efg	0.76±0.025 e	0.85±0.021 efg	3.77±0.021 bc	0.71±0.016 ab	0.94±0.029 ab	0.98±0.021 a
Jy1	0.59±0.039 e	1.05±0.033 g	1.66d±0.057 d	1.03±0.021 hi	2.12±0.024 b	1.31±0.071 ab	4.61±0.062 b	0.74±0.033 ab	0.87±0.012 abc	0.89±0.012 ab
Ymt2188	1.11±0.080 cd	1.09±0.020 efg	0.56±0.070 i	1.82±0.017 bc	1.01±0.050 d	1.27±0.155 b	1.22±0.049 e	0.52±0.026 c	0.80±0.088 c	0.85±0.057 b
QsF1	1.57±0.078 b	1.08±0.045 efg	1.11±0.012 fg	1.66±0.086 cde	1.17±0.046 d	0.67±0.087 fg	3.68±0.050 bc	0.67±0.045 ab	0.85±0.034 abc	0.82±0.057 b
Jyys	0.96±0.069 d	1.51±0.091 bc	1.20±0.066 fg	1.25±0.033 fgh	1.24±0.008 d	0.90±0.021 def	3.36±0.109 c	0.70±0.057 ab	0.89±0.033 abc	0.85±0.005 b
Msjp313	1.59±0.091 b	1.21±0.086 defg	0.80±0.029 hi	1.86±0.036 bc	1.03±0.033 d	1.17±0.070 bcd	1.05±0.037 e	0.60±0.049 bc	0.87±0.037 abc	0.86±0.037 b
均值Mean	1.13	1.34	1.50	1.55	1.35	1.04	3.25	0.65	0.87	0.87
标准差SD	0.43	0.44	0.78	0.38	0.58	0.28	1.49	0.08	0.06	0.07
变异系数CV/%	38.05	32.84	52.00	24.52	42.96	26.92	45.85	12.31	6.90	8.04

Table 3 Correlation analysis of 10 wet?cold tolerance identification indexes

指标Index	过氧化氢酶CAT	苯丙氨酸解氨酶PAL	多酚氧化酶PPO	超氧阴离子O $2 -$	谷胱甘肽GSH	可溶性糖SS	脯氨酸 Pro	株高 SH	茎粗 SD
苯丙氨酸解氨酶PAL	-0.175
多酚氧化酶PPO	-0.258	0.504^**
超氧阴离子O $2 -$	0.397^*	-0.053	0.178
谷胱甘肽GSH	-0.395^*	v0.414^*	-0.564^**	-0.519^**
可溶性糖SS	-0.379^*	-0.181	-0.022	-0.141	0.16
脯氨酸Pro	-0.794^**	0.036	-0.144	-0.579^*	0.442^**	0.125
株高SH	-0.661^**	0.032	0.070	-0.505^**	0.457^**	0.269	0.796^**
茎粗SD	-0.528^**	0.274	-0.228	0.055	0.411^*	0.303	0.524^**	0.498^*
叶长LL	-0.524^**	0.304	0.312	0.259	0.407^*	0.286	0.204	0.407^*	0.462^*

Table 3 Correlation analysis of 10 wet?cold tolerance identification indexes

指标Index	过氧化氢酶CAT	苯丙氨酸解氨酶PAL	多酚氧化酶PPO	超氧阴离子O $2 -$	谷胱甘肽GSH	可溶性糖SS	脯氨酸 Pro	株高 SH	茎粗 SD
苯丙氨酸解氨酶PAL	-0.175
多酚氧化酶PPO	-0.258	0.504^**
超氧阴离子O $2 -$	0.397^*	-0.053	0.178
谷胱甘肽GSH	-0.395^*	v0.414^*	-0.564^**	-0.519^**
可溶性糖SS	-0.379^*	-0.181	-0.022	-0.141	0.16
脯氨酸Pro	-0.794^**	0.036	-0.144	-0.579^*	0.442^**	0.125
株高SH	-0.661^**	0.032	0.070	-0.505^**	0.457^**	0.269	0.796^**
茎粗SD	-0.528^**	0.274	-0.228	0.055	0.411^*	0.303	0.524^**	0.498^*
叶长LL	-0.524^**	0.304	0.312	0.259	0.407^*	0.286	0.204	0.407^*	0.462^*

Table 4 Principal component analysis and eigenvalue

指标 Index	第一主成分PC1 （X₁）	第二主成分PC2（X₂）	第三主成分PC3（X₃）	第四主成分PC4（X₄）
株高SH（I₁）	0.876	0.275	-0.067	-0.173
茎粗SD（I₂）	0.751	0.274	0.431	0.200
叶长LL（I₃）	0.671	-0.015	0.146	0.387
过氧化氢酶CAT（I₄）	-0.481	0.112	0.623	-0.512
谷胱甘肽GSH（I₅）	0.292	0.784	0.017	-0.084
脯氨酸Pro（I₆）	0.840	0.242	-0.137	-0.206
多酚氧化酶PPO（I₇）	0.406	-0.464	-0.593	-0.155
可溶性糖SS（I₈）	-0.433	0.437	-0.430	0.571
超氧阴离子O $2 -$ （I₉）	0.140	-0.395	0.520	0.572
苯丙氨酸解氨酶PAL（I₁₀）	0.621	-0.616	0.047	-0.124
特征值Eigenvalue	3.560	1.779	1.429	1.224
贡献率Contribution rate/%	36.601	17.786	14.285	12.240
累计贡献率Accumulative contribution rate/%	36.601	54.387	68.672	80.912

Table 4 Principal component analysis and eigenvalue

指标 Index	第一主成分PC1 （X₁）	第二主成分PC2（X₂）	第三主成分PC3（X₃）	第四主成分PC4（X₄）
株高SH（I₁）	0.876	0.275	-0.067	-0.173
茎粗SD（I₂）	0.751	0.274	0.431	0.200
叶长LL（I₃）	0.671	-0.015	0.146	0.387
过氧化氢酶CAT（I₄）	-0.481	0.112	0.623	-0.512
谷胱甘肽GSH（I₅）	0.292	0.784	0.017	-0.084
脯氨酸Pro（I₆）	0.840	0.242	-0.137	-0.206
多酚氧化酶PPO（I₇）	0.406	-0.464	-0.593	-0.155
可溶性糖SS（I₈）	-0.433	0.437	-0.430	0.571
超氧阴离子O $2 -$ （I₉）	0.140	-0.395	0.520	0.572
苯丙氨酸解氨酶PAL（I₁₀）	0.621	-0.616	0.047	-0.124
特征值Eigenvalue	3.560	1.779	1.429	1.224
贡献率Contribution rate/%	36.601	17.786	14.285	12.240
累计贡献率Accumulative contribution rate/%	36.601	54.387	68.672	80.912

Table 5 Index of each variety and ranking

品种 Variety	X₁	X₂	X₃	X₄	U（X₁）	U（X₂）	U（X₃）	U（X₄）	D	排序Ranking
权重 W	—	—	—	—	0.446	0.223	0.179	0.152	—	—
QsF1	6.989	2.041	-1.475	-0.335	0.369	0.663	0.659	0.664	0.531	8
Syz	5.887	0.006	0.030	-0.195	0.371	0.340	0.369	0.342	0.359	13
Hl35	6.663	-1.327	-1.609	0.131	0.445	0.340	0.357	0.709	0.446	10
Bn5	7.375	1.813	-0.969	0.133	0.666	0.646	0.666	0.336	0.611	1
Zn26	8.234	2.245	-0.743	-0.468	0.584	0.351	0.660	0.646	0.555	6
Sy4	6.026	1.694	0.487	0.921	0.361	0.436	0.397	0.608	0.422	11
Jy1	2.826	1.601	0.880	-0.959	0.592	0.625	0.639	0.450	0.586	3
Jy64	1.770	2.087	-0.233	1.597	0.617	0.594	0.646	0.509	0.601	2
Jyys	4.958	1.305	1.817	0.289	0.663	0.543	0.438	0.445	0.563	4
Ymt2188	4.449	-0.530	-0.490	1.043	0.426	0.597	0.657	0.666	0.542	7
Ccmc	7.477	-0.774	-0.562	0.110	0.654	0.430	0.663	0.346	0.559	5
Msjp313	2.073	1.079	1.618	0.948	0.350	0.480	0.347	0.358	0.380	12
FyF1	5.425	0.445	-0.400	0.120	0.506	0.395	0.625	0.548	0.508	9

Fig. 1 Cluster analysis of 13 cucumber varieties based on D valueNote： The abbreviations of varieties are same to Table 1.

Table 6 Relative values of indexes of 7 tested varieties

指标 Index	Jy41	Jy4	Zn28	Jy35	Rg2	Sy11	Drt8-9F
株高SH	0.532	0.62	0.530	0.623	0.515	0.667	0.243
茎粗SD	0.759	0.814	0.746	0.793	0.790	0.855	0.608
叶长LL	0.892	0.814	0.277	0.462	0.368	0.781	0.663
过氧化氢酶活性CAT	0.418	0.155	0.468	0.488	0.467	0.580	3.574
谷胱甘肽含量GSH	0.627	1.804	1.074	1.069	1.341	2.908	1.569
脯氨酸含量Pro	2.069	2.489	3.685	1.783	3.798	3.629	1.322
多酚氧化酶活性PPO	1.258	0.743	2.507	0.657	1.117	1.003	0.958
可溶性糖含量SS	1.896	2.703	1.311	1.480	2.021	1.781	1.996
超氧阴离子含量O $2 -$	1.524	2.252	1.339	0.977	1.016	2.949	0.744
苯丙氨酸解氨酶活性PAL	1.602	0.716	0.883	0.978	0.985	1.401	2.687

Table 6 Relative values of indexes of 7 tested varieties

指标 Index	Jy41	Jy4	Zn28	Jy35	Rg2	Sy11	Drt8-9F
株高SH	0.532	0.62	0.530	0.623	0.515	0.667	0.243
茎粗SD	0.759	0.814	0.746	0.793	0.790	0.855	0.608
叶长LL	0.892	0.814	0.277	0.462	0.368	0.781	0.663
过氧化氢酶活性CAT	0.418	0.155	0.468	0.488	0.467	0.580	3.574
谷胱甘肽含量GSH	0.627	1.804	1.074	1.069	1.341	2.908	1.569
脯氨酸含量Pro	2.069	2.489	3.685	1.783	3.798	3.629	1.322
多酚氧化酶活性PPO	1.258	0.743	2.507	0.657	1.117	1.003	0.958
可溶性糖含量SS	1.896	2.703	1.311	1.480	2.021	1.781	1.996
超氧阴离子含量O $2 -$	1.524	2.252	1.339	0.977	1.016	2.949	0.744
苯丙氨酸解氨酶活性PAL	1.602	0.716	0.883	0.978	0.985	1.401	2.687

Table 7 Evaluation accuracy of equation

品种Variety	原始值D	预测值P	差值Difference	准确度Accuracy/%
Jy41	0.439	0.457	0.018	95.9
Jy4	0.528	0.537	0.009	98.3
Zn28	0.560	0.598	0.038	93.2
Jy35	0.515	0.490	-0.025	95.1
Rg2	0.494	0.522	0.028	94.3
Sy11	0.460	0.430	-0.030	93.5
Drt8-9F	0.556	0.526	-0.030	94.6

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