Genome Sequencing Application on Molecular Characterization Assessment of Transgenic Plant

doi:10.13304/j.nykjdb.2019.0824

Journal of Agricultural Science and Technology ›› 2020, Vol. 22 ›› Issue (5): 6-14.DOI: 10.13304/j.nykjdb.2019.0824

Previous Articles Next Articles

Genome Sequencing Application on Molecular Characterization Assessment of Transgenic Plant

MA Shuo1, JIAO Yue2, WANG Xujing1, ZHAI Yong2, WANG Zhixing1*

1.MARA Key Laboratory on Safety Assessment(Molecular) of Agri-GMO, Ministry of Agriculture and Rural Affairs; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2.Development Center for Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100122, China

Received:2019-10-09 Online:2020-05-15 Published:2019-12-18

高通量测序技术在转基因植物分子特征评价中的应用

马硕1,焦悦2,王旭静1,翟勇2,王志兴1*

1.中国农业科学院生物技术研究所, 农业农村部农业转基因生物安全评价（分子）重点实验室, 北京 100081; 2.农业农村部科技发展中心, 北京 100122

通讯作者: *通信作者王志兴 Email: wangcotton@126.com
作者简介:马硕 Email: mashuo0801@163.com；
基金资助:
农产品质量安全监管经费项目。

Abstract

Abstract: Molecular characterization is an important basis for selecting and identifying transgenic event, and also the main content which must be provided for safety assessment of transgenic plant. The development of DNA sequencing technology provides a new strategy for accurately analyzing the molecular characterization of transgenic plants. This paper reviewed the development and technical principle of DNA sequencing technology and the application of high-throughput sequencing in the analysis of molecular characterization. In addition, it described the requirements of European Union and Canada for sequencing data provided for the safety assessment of transgenic plant. Based on above, adopt high-throughput sequencing data in the safety assessment of transgenic plant in China were proposed, which provided theoretical support for the application of high-throughput sequencing technology in the safety assessment of transgenic plant in China.

Key words: genome sequencing, transgenic plant, molecular characterization, safety assessment

摘要： 分子特征是筛选和鉴别转化体重要依据，也是转基因植物安全评价必须提供的内容。DNA测序技术的发展为精准解析转基因植物的分子特征提供了新的技术方案。综述了高通量测序技术在转基因植物分子特征中的应用及欧盟和加拿大对转基因植物安全评价中所提供测序数据的要求，并提出了我国转基因植物安全评价中采纳测序数据时的建议，为高通量测序技术在我国转基因生物安全评价中的应用提供理论支撑。

关键词: 基因组测序, 转基因植物, 分子特征, 安全评价

MA Shuo1, JIAO Yue2, WANG Xujing1, ZHAI Yong2, WANG Zhixing1*. Genome Sequencing Application on Molecular Characterization Assessment of Transgenic Plant[J]. Journal of Agricultural Science and Technology, 2020, 22(5): 6-14.

马硕1,焦悦2,王旭静1,翟勇2,王志兴1*. 高通量测序技术在转基因植物分子特征评价中的应用[J]. 中国农业科技导报, 2020, 22(5): 6-14.

[1]	Guoqing LU, Caixia MA, Guoqing SUN, Huiming GUO, Hongmei CHENG. Molecular Characterization and Inheritance Stability Analysis of Herbicide-resistant Cotton GV-2 [J]. Journal of Agricultural Science and Technology, 2023, 25(1): 42-49.
[2]	Junjie ZOU, Miaoyun XU, Lan ZHANG, Yanzhong LUO, Yuan LIU, Hongyan ZHENG, Lei WANG. Molecular Characterizations of Stacked Transgenic Maize BBHTL8-1 with Insect Resistance， Glyphosate Tolerance and Improved Quality [J]. Journal of Agricultural Science and Technology, 2022, 24(2): 77-85.
[3]	Junjie ZOU, Miaoyun XU, Lan ZHANG, Hongyan ZHENG, Lei WANG. Molecular Characteristics and Functional Evaluation of Transgenic Maize BFL4-1 With Stacked Insect and Herbicide Resistance Traits [J]. Journal of Agricultural Science and Technology, 2022, 24(1): 31-37.
[4]	WU Shan, PANG Junqin, ZHUANG Junhong, CHEN Limei*. Research, Development, and Safety Administration of Genetically Modified Crops in China [J]. Journal of Agricultural Science and Technology, 2020, 22(11): 11-16.
[5]	JIAO Yue1, WU Gang2, HUANG Yaohui1, XIONG Li1, ZHAI Yong1*. Genome Editing Technology and Its Safety Assessment Management [J]. Journal of Agricultural Science and Technology, 2018, 20(4): 12-19.
[6]	QI Xiaozhe, HUANG Kunlun*. Research Progress on Safety Assessment of Genetically Modified Food [J]. , 2013, 15(4): 14-19.
[7]	ZHAO Jie, LIU Zhi, PENG Yu-fa, QI Fang-jun. Progress on Technology for Detection and Evaluation of Unintended Effects of Genetically Modified Plants [J]. , 2013, 15(2): 64-69.
[8]	LIU Xiao-qing, CHEN Ru-mei. Strategies for Enhancing Expression of Foreign Genes in Transgenic Plant [J]. , 2012, 14(1): 76-84.
[9]	GUO Qian-qian1, SUN Xi-sen2, TANG Yi-xiong1, WU Yan-min1. Applications of Phosphomannose Isomerase-based Selection System in Transgenic Plants [J]. , 2011, 13(6): 12-19.
[10]	ZHANG Yong-xia1,2, LANG Zhi-hong1, ZHU Li1, YANG Li-mei3, ZHANG Jie4, . Studies on Genetic Transformation Bt cry1Ⅰa Gene into Cauliflower by Agrobacterium-mediated Transformation [J]. , 2011, 13(1): 15-19.
[11]	ZHOU Zhang-fa, ZHANG Rui, ZHANG Xiao, LUO Shu-ping, GUO San-dui. Technological Improvement of Southern Blot Using Digoxigenin-labeled Probes with Random-primed Method [J]. , 2009, 11(4): 123-128.
[12]	YAN Yong-liang\| YANG Jian, DOU Yue-tan\| PING Shu-zhen\| WANG Yi-ping, JIN Qi, . Genome Structure and Functional Annotation of Nitrogen- \|fixing Pseudomonas stutzeri A1501 [J]. , 2008, 10(5): 45-52.
[13]	LIU Dan, LIU Gui-hua, WANG Han-zhong. Isolation of Resistant Genes and Their Gene Engineering Research Developments in Rapeseed [J]. , 2008, 10(3): 6-11.
[14]	CHEN Yu-hui, ZHA0 Ling-xia, CUI Li-jie, TANG Ke-xuan . Progress in Transgenic Plants Producing Pharmaceutical Proteins [J]. , 2007, 9(5): 36-44.
[15]	WANG Xiao-xue, GUO Jun, TIAN Jian\|WU Ning-feng, FAN Yun-liu . Expression of a Bacterial Organophosphorus Hydrolase in Transgenic Tobacco Plants [J]. , 2007, 9(4): 108-110.

Genome Sequencing Application on Molecular Characterization Assessment of Transgenic Plant

高通量测序技术在转基因植物分子特征评价中的应用

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics