Abstract:

A method for determination of urea in feed was estabilished.Urea was extracted by potassium hexacyanoferrate and zinc acetate,determined by colorimetry.The linear range of the method was among 0.1~10 mg/mL,with correlation coefficient was 0.999 9.The concentration limit of quantity was 0.1%.In order to validate accuracy and precision of the method,several kinds of reference feeds such as cow concentrate supplement,sheep concentrate supplement,cow concentrate,sheep concentrate and beef concentrate,were prepa...

Key words: feed, urea, colorimetry;

摘要：

建立了饲料中尿素的测定方法。经亚铁氰化钾和乙酸锌提取饲料中的尿素,用比色法测定。尿素在0.1~10 mg/mL范围内线性关系良好,回归方程为Abs=0.056 82×C-0.001 41,相关系数r=0.999 9,最低定量限为0.1%。为了验证方法的准确度、精密度,配制了不添加尿素的奶牛精补料、羊精补料、奶牛浓缩料、羊浓缩料、肉牛浓缩料等空白饲料,对不同饲料进行了多浓度、多次的添加回收实验,平均回收率均在80.0%~120.0%范围内,RSD均小于10.0%。

关键词: 饲料 尿素 比色法