Comparative Study on Pancreatic Lipase Inhibitory Activity and Antioxidant Activity of Gingerol Before and After Purification

doi:10.13304/j.nykjdb.2021.0855

Abstract

Abstract:

In order to explore the purification method of gingerol by macroporous resin， four different polar resins （NKA-9， AB-8， D4020 and D101） were selected to study the static adsorption-desorption kinetics. The results showed that AB-8 was the best resin which was used as adsorbent. The optimum purification process of gingerol was discussed， and the scavenging ability of gingerol to 1,1-diphenyl-2-trinitrophenylhydrazine （DPPH） radical， 2,2-diazo-bis（3-ethyl-benzothiazole-6-sulfonic acid） diammonium （ABTS） radical and its inhibitory activity to pancreatic lipase were compared before and after purification. The contents of 6-gingerol， 8-gingerol and 10-gingerol in the crude extract before purification and after purification were determined by HPLC. The results showed that the optimum conditions for dynamic purification of gingerol by AB-8 resin were as follows： sample mass concentration 1.6 mg·mL^-1， pH=4， sample flow rate 1.5 mL·min^-1， elution rate 1.5 mL·min^-1 at 80% ethanol solution. The purity of gingerol after purification was increased by 3.3 times. The results of high performance liquid chromatography showed that the content of three gingerol monomers was more than 3 times higher than that before purification. The inhibition rate and free radical scavenging ability of purified gingerol on porcine pancreatic lipase were significantly higher than those before purification， indicating that AB-8 resin had a good effect on the separation and purification of gingerol and could achieve the enrichment of components， which was of great significance to improve the added value of gingerol utilization.

Key words: macroporous resin, gingerol, adsorption, desorption, antioxidation, inhibition of pancreatic lipase

摘要：

为了探讨姜酚的大孔树脂纯化方法，选择4种不同极性树脂（NKA-9、AB-8、D4020 和D101）作静态吸附-解吸动力学研究。结果表明，AB-8为最佳树脂，以AB-8树脂为吸附剂，对其最佳纯化工艺进行探讨，并比较纯化前后姜酚对1，1-二苯基-2-三硝基苯肼（1，1-diphenyl-2-trinitrophenylhydrazine， DPPH）自由基、2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（2，2-diazo-bis （3-ethyl-benzothiazole-6-sulfonic acid） diammonium，ABTS）自由基等的清除能力和对胰脂肪酶抑制活性，采用高效液相色谱法测定纯化前粗提物和纯化后纯化物中6-姜酚、8-姜酚、10-姜酚含量。结果表明，AB-8树脂动态纯化姜酚最佳条件为：上样质量浓度1.6 mg·mL^-1，pH=4，上样流速1.5 mL·min^-1，以80%乙醇溶液流速1.5 mL·min^-1洗脱，经纯化后姜酚纯度为纯化前的3.3倍，高效液相色谱分析结果表明3种姜酚类单体物质含量相比纯化前提高了3倍以上，纯化后的姜酚对猪胰脂肪酶的抑制率和自由基清除能力明显高于纯化前，说明AB-8树脂对姜酚有较好的分离纯化作用，能够实现成分的富集，对提高姜酚的利用附加值具有重要意义。

关键词: 大孔树脂, 姜酚, 吸附, 解吸, 抗氧化, 抑制胰脂肪酶

CLC Number:

Q946

Jing XU, Xiufen WANG, Xiaoyan LIU. Comparative Study on Pancreatic Lipase Inhibitory Activity and Antioxidant Activity of Gingerol Before and After Purification[J]. Journal of Agricultural Science and Technology, 2023, 25(5): 96-105.

徐菁, 王秀芬, 刘晓燕. 姜酚纯化前后对胰脂肪酶抑制活性、抗氧化活性比较研究[J]. 中国农业科技导报, 2023, 25(5): 96-105.

Figures/Tables 13

References 26

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时间Time/min	流动相Mobile phase
时间Time/min	A（0.1%磷酸溶液） A（0.1% phosphoric acid）/%	B（乙腈） B（Acetonitrile）/%
0.00	14	86
7.00	12	88
17.50	10	90

时间Time/min	流动相Mobile phase
时间Time/min	A（0.1%磷酸溶液） A（0.1% phosphoric acid）/%	B（乙腈） B（Acetonitrile）/%
0.00	14	86
7.00	12	88
17.50	10	90

组号 Group number	样品 Sample	胰脂肪酶 Pancreatic lipase	对硝基苯磷酸二钠六水合物 PNPP	Tris-盐酸缓冲液 Tris-HCl
A₁	30	100	100	70
A₀	30	0	100	170
B₁	0	100	100	100
B₀	0	0	100	200

组号 Group number	样品 Sample	胰脂肪酶 Pancreatic lipase	对硝基苯磷酸二钠六水合物 PNPP	Tris-盐酸缓冲液 Tris-HCl
A₁	30	100	100	70
A₀	30	0	100	170
B₁	0	100	100	100
B₀	0	0	100	200

树脂型号 Resin type	方程类型 Equation type	拟合方程 Fitting equation	R²	动力学参数 Kinetic parameter
AB-8	一级动力学 First-order dynamic	In（Q_e-Q_t）=0.640-0.852t	0.876 9	Q_e=4.13 mg·g^-1， K₂=1.17
	二级动力学 Second-order dynamic	t/Q_t=0.050+0.242t	0.999 7
	颗粒内扩散 diffusion particle	Q_t=0.302t^1/2 +3.209	0.684 7
D101	一级动力学 First-order dynamic	In（Q_e-Q_t）=-0.572-0.807t	0.866 2
	二级动力学 Second-order dynamic	t/Q_t=0.101+0.244t	0.999 0	Q_e=4.09 mg·g^-1， K₂=0.59
	颗粒内扩散 diffusion particle	Q_t =0.487t^1/2 +2.572	0.714 3
D4020	一级动力学 First-order dynamic	In（Q_e-Q_t）=1.284-1.019t	0.862 6	Q_e=4.03 mg·g^-1， K₂=0.42
	二级动力学 Second-order dynamic	t/Q_t=0.248+0.146t	0.998 2
	颗粒内扩散 diffusion particle	Q_t=0.592t^1/2 +2.147	0.729 8
NKA-9	一级动力学 First-order dynamic	In（Q_e-Q_t）=0.768-0.952t	0.868 2	Q_e=3.25 mg·g^-1， K₂=0.71
	二级动力学 Second-order dynamic	t/Q_t=0.133+0.307t	0.999 3
	颗粒内扩散 diffusion particle	Q_t=0.388t^1/2 +2.325	0.759 0