Artificial Gynogenesis Technique and Its Application in Fish Breeding

doi:10.13304/j.nykjdb.2021.0576

Abstract

Abstract:

Gynogenesis is a special reproductive mode that depends on female prokaryotic development. Artificial gynogenesis technique （AGT） has been developed into an effective way of fish genetic improvement， which plays an important role in the purification and rejuvenation of fish germplasm and breeding of fine species. Based on the practice of fish breeding， this paper systematically summarized the technical approaches and research status of AGT， and put forward the existing problems of AGT， in order to provide reference for the future development of AGT.

Key words: fishes, artificial gynogenesis, breeding

摘要：

雌核生殖是一种依靠雌原核发育的特殊生殖方式，人工雌核生殖已经发展成为鱼类遗传改良的有效途径，在鱼类种质提纯复壮、良种选育等方面发挥重要作用。从鱼类育种的实践出发，系统总结了鱼类人工雌核生殖的技术途径、研究现状，提出了鱼类人工雌核生殖技术存在的问题，旨在为鱼类人工雌核生殖技术未来发展提供参考。

关键词: 鱼类, 人工雌核生殖, 育种

CLC Number:

S961

Wen FU, Liangyue PENG, Yamei XIAO. Artificial Gynogenesis Technique and Its Application in Fish Breeding[J]. Journal of Agricultural Science and Technology, 2022, 24(2): 42-48.

符文, 彭亮跃, 肖亚梅. 人工雌核生殖技术及其在鱼类育种中的应用[J]. 中国农业科技导报, 2022, 24(2): 42-48.

References 58

1	KOMEN H， THORGAARD G H. Androgenesis， gynogenesis and the production of clones in fishes： a review ［J］. Aquaculture， 2007， 269（1-4）：150-173.
2	HERTWIG O. Die Radiumkrankheit tierischer Keimzellen ［J］. Archiv für Mikroskopische Anatomie， 1911，77（1）：97A-164A.
3	DARNELL R M， ABRAMOFF P. Distribution of the gynogenetic fish， Poeciliaformosa， with remarks on the evolution of the species ［J］. Copeia， 1968， 1968（2）：354-361.
4	MENZEL B W， DARNELL R M. Systematics of Poeciliamexicana （Pisces： Poeciliidae） in Northern Mexico ［J］. Copeia， 1973， 1972（2）：225-237.
5	俞豪祥. 银鲫雌核发育的细胞学观察［J］. 水生生物学报，1982，7（4）：481-487.
	YU H X. A cytological observation on gynogenesis of crucian carp （Carassiusauratusgibelio）［J］. Acta Hydrob. Sin.， 1982， 7（4）：481-487.
6	FAN Z， SHEN J. Studies on the evolution of bisexual reproduction in crucian carp （Carassiusauratusgibelio Bloch）［J］. Aquaculture， 1990， 84（3-4）：235-244.
7	杨兴棋，陈敏容．江西彭泽鲫生殖方式的初步研究［J］. 水生生物学报，1992，16（3）：277-280.
	YANG X Q， CHEN M R. Preliminary studies on the mode of reproduction in crucian carp （Carassiusauratus） of pengze ［J］. Acta Hydrob. Sin.， 1992， 16（3）：277-280.
8	SCHLUPP I. The evolutionary ecology of gynogenesis ［J］. Annu. Rev. Ecol. Evol. Syst.， 2005， 36：399-417.
9	葛伟，单仕新，蒋一珪．雌核发育银鲫的受精生物学研究——天然雌核发育银鲫繁殖方式的讨论［J］. 水生生物学报，1992，16（2）：3-6，99.
	GE W， SHAN S X， JIANG Y G. Fertilization biology of gynogenetic crucian carp （Carassiusauratusgibelio）， with a discussion on the reproductive modes of the naturally gynogenetic crucian carp ［J］. Acta Hydrob. Sin.， 1992， 16（2）：3-6，99.
10	AVISE J C， TREXLER J C， TRAVIS J， et al.. Poecilia mexicana is the recent female parent of the unisexual fish P. formosa ［J］. Evolution， 1991， 45（6）：1530-1533.
11	岳振宇，蒋一珪，单仕新．天然雌核发育银鲫卵子控制异源精核发育的受精学机制［J］. 水生生物学报，1996，20（3）：236-241.
	YUE Z Y， JIANG Y G， SHAN S X. The regulated development of heterologous sperm nucleus by eggs of natural gynogenetic silver crucian carp （Carassiusauratusgibelio） during fertilization ［J］. Acta Hydrob. Sin.， 1996， 20（3）：236-241.
12	STANLEY J G， SNEED K E. Artificial gynogenesis and its application in genetics and selective breeding of fishes ［M］// BLAXTER J H S. The Early Life History of Fish. Berlin， Heidelberg： Springer， 1974： 527-536.
13	FELIP A， ZANUY S， CARRILLO M， et al.. Induction of triploidy and gynogenesis in teleost fish with emphasis on marine species ［J］. Genetica， 2001， 111（1）：175-195.
14	徐康，段巍，肖军，等．鱼类遗传育种中生物学方法的应用及研究进展［J］. 中国科学：生命科学，2014，44（12）：1272-1288.
	XU K， DUAN W， XIAO J， et al.. Development and application of biological technologies in fish genetic breeding ［J］. Sci. Chin. Life Sci.， 2014， 44（12）：1272-1288.
15	JI X S， TIAN Y S， YANG J F， et al.. Artificial gynogenesis in Cynoglossussemilaevis with homologous sperm and its verification using microsatellite markers ［J］. Aquac. Res.， 2010， 41（6）：913-920.
16	PERUZZI S， CHATAIN B. Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass， Dicentrarchuslabrax L．： relative efficiency of methods and parental variability ［J］. Aquaculture， 2000， 189（1-2）：23-37.
17	CHEN R， LOU B， XU D， et al.. Induction of meiotic gynogenesis in yellow drum （Nibeaalbiflora， Sciaenidae） using heterologous sperm and evidence for female homogametic sex determination ［J］. Aquaculture， 2017， 479：667-674.
18	SABER M H， NOVEIRI S B， POURKAZEMI M， et al.. Induction of meiotic gynogenesis in ship sturgeon Acipensernudiventris using UV-irradiated heterologous sperm ［J］. J. Appl. Genet.， 2014， 55（2）：223-229.
19	STANLEY J G. Production of hybrid， androgenetic， and gynogenetic grass carp and carp ［J］. Trans. Am. Fish. Soc.， 1976， 105（1）：10-16.
20	ZHANG H， LIU S J， ZHANG C， et al.. Induced gynogenesis in grass carp （Ctenopharyngodonidellus） using irradiated sperm of allotetraploid hybrids ［J］. Mar. Biotechnol.， 2011， 13（5）：1017-1026.
21	LI T， JIA Y， GAO J， et al.. Optimization of shock conditions to induce gynogenesis in topmouth culter （Culteralburnus）［J］. Aquaculture， 2018， 490：344-349.
22	GONG D， XU L， WU C， et al.. Two types of gynogenetic blunt snout bream derived from different sperm ［J/OL］. Aquaculture， 2019， 511： 734250 ［2021-09-28］. .
23	CHEN F， LI X Y， ZHOU L， et al.. Stable genome incorporation of sperm-derived DNA fragments in gynogenetic clone of gibel carp ［J］. Mar. Biotechnol.， 2020， 22（1）：54-66.
24	MAO Z， FU Y， WANG Y， et al.. Correction to： evidence for paternal DNA transmission to gynogenetic grass carp ［J/OL］. BMC Genet.， 2020， 21（1）：56 ［2021-09-28］. .
25	MAO Z， FU Y， WANG S， et al.. Further evidence for paternal DNA transmission in gynogenetic grass carp ［J］. Sci. Chin. Life Sci.， 2020， 63（9）：1287-1296.
26	SUN Y D， ZHANG C， LIU S J， et al.. Induction of gynogenesis in Japanese crucian carp （Carassiuscuvieri）［J］. Acta Genet. Sin.， 2006， 33（5）：405-412.
27	YI M S， LI Y Q， LIU J D， et al.. Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp， Carassiusauratusgibelio Bloch ［J］. Chromosome Res.， 2003， 11（7）：665-671.
28	ZHAO J， LIU L G， CHEN X L， et al.. Karyotypic analysis of the multiple tetraploid allogynogenetic pengze crucian carp and its parents ［J］. Aquaculture， 2004， 237（1-4）：117-129.
29	吴仲庆．水产生物遗传育种学［M］. 福建厦门：厦门大学出版社，2000：1-321.
30	YOUSEFIAN M， AMIRINIA C， BERCSENYI M， et al.. Induced mitotic gynogenesis in common carp （Cyprinuscarpio L.）， optimizing irradiation dose of X-and gamma-ray ［J］. Allattenyesztes es Takarmanyozas （Hungary）， 1997， 46（2）：97-105.
31	ONOZATO H. The “Hertwig effect” and gynogenesis in chum salmon Oncorhynchusketa eggs fertilized with ⁶⁰Co gamma-ray irradiated milt ［J］. Bull. Jap. Soc. Sci. Fish.， 1982， 48（9）：1237-1244.
32	IJIRI K I. Gamma-ray irradiation of the sperm of the fish Oryziaslatipes and induction of gynogenesis ［J］. J. Radiat. Res.， 1980， 21（3-4）：263-270.
33	CIEMINIS K G K， RANČELIENĖ V M， PRIJALGAUSKIENĖ A J， et al.. Chromosome and DNA damage and their repair in higher plants irradiated with short-wave ultraviolet light ［J］. Mutation Res. Fundam. Mol. Mech. Mutagenesis， 1987，181（1）：9-16.
34	KOMEN J， DUYNHOUWER J， RICHTER C J J， et al.. Gynogenesis in common carp （Cyprinuscarpio L．）： I. Effects of genetic manipulation of sexual products and incubation conditions of eggs ［J］. Aquaculture， 1988， 69（3-4）：227-239.
35	PALTI Y， LI J J， THORGAARD G H. Improved efficiency of heat and pressure shocks for producing gynogenetic rainbow trout ［J］. Prog. Fish-Culturist， 1997， 59（1）：1-13.
36	吴清江，陈荣德，叶玉珍，等．鲤鱼人工雌核发育及其作为建立近交系新途径的研究［J］. 遗传学报，1981，8（1）：50-55.
	WU Q J， CHEN R D， YE Y Z， et al.. Investigation on the carp gynogenesis with reference to establihing a pure line ［J］. Acta Genet. Sin.， 1981，8（1）：50-55.
37	REFSTIE T. Tetraploid rainbow trout produced by cytochalasin B ［J］. Aquaculture， 1981， 25（1）：51-58.
38	VIDAIR C A， DOXSEY S J， DEWEY W C. Heat shock alters centrosome organization leading to mitotic dysfunction and cell death ［J］. J. Cell. Physiol.， 1993， 154（3）：443-455.
39	DUSTIN P. Microtubules ［M］. Springer Science & Business Media， 2012：1-452.
40	BENCSIK I， PACALA N， CAPRITA R， et al.. Diploid gynogenesis induced by heat shock after activation with UV-irradiated sperm in rainbow trout （Oncorhynchusmykiss）［J］. Sci. Papers Anim. Sci. Biotechnol.， 2011， 44（1）：162-165.
41	MORGAN A J， MURASHIGE R， WOOLRIDGE C A， et al.. Effective UV dose and pressure shock for induction of meiotic gynogenesis in southern flounder （Paralichthyslethostigma） using black sea bass （Centropristisstriata） sperm ［J］. Aquaculture， 2006， 259（1-4）：290-299.
42	LOU Y D， PURDOM C E. Diploid gynogenesis induced by hydrostatic pressure in rainbow trout， Salmogairdneri Richardson ［J］. J. Fish Biol.， 1984， 24（6）：665-670.
43	GHIGLIOTTI L， BOLLA S L， DUC M， et al.. Induction of meiotic gynogenesis in Atlantic cod （Gadusmorhua L．） through pressure shock ［J］. Anim. Reprod. Sci.， 2011， 127（1-2）：91-99.
44	LUO H， FENG K， CHEN J， et al.. Telophase of the first cleavage is the key stage for optimally inducing mitotic gynogenesis in rice field eel （Monopterusalbus）［J/OL］. Aquaculture， 2020， 523：735241 ［2021-09-28］. .
45	胡炜，罗红瑞，冯科，等．一种控制黄鳝受精卵第一次卵裂的方法及其应用： ZL201711265194.8 ［P］. 2020-09-11.
46	刘海金，侯吉伦，刘奕．牙鲆雌核发育研究进展［J］. 中国水产科学，2017，24（4）：902-912.
	LIU J H， HOU J L， LIU Y. Gynogenesis in Japanese flounder： a review ［J］. J. Fish. Sci. Chin.， 2017， 24（4）：902-912.
47	QIN Q B， WANG J， WANG Y D， et al.. Organization and variation analysis of 5S rDNA in gynogenetic offspring of Carassius auratus red var.（♀）× Megalobrama amblycephala （♂）［J/OL］. BMC Genet.， 2015， 16（1）：［2021-09-28］. .
48	WANG S， YE X， WANG Y， et al.. A new type of homodiploid fish derived from the interspecific hybridization of female common carp× male blunt snout bream ［J/OL］. Sci. Rep.， 2017， 7（1）：4189［2021-09-28］. .
49	WU C， HUANG X， HU F， et al.. Production of diploid gynogenetic grass carp and triploid hybrids derived from the distant hybridization of female grass carp and male topmouth culter ［J］. Aquaculture， 2019， 504：462-470.
50	LUO K K， XIAO J， LIU S J， et al.. Massive production of all-female diploids and triploids in the crucian carp ［J］. Int. J. Biol. Sci.， 2011， 7（4）：487-495.
51	肖亚梅，彭亮跃，龙敏笛，等．一种快速培育多能干细胞荧光标记斑马鱼纯合个体的方法： CN201911358182.9 ［P］. 2021-04-13.
52	李志，李熙银，汪洋，等．一种高效创制黄河鲶mstn基因突变体的方法： CN202011237170.3 ［P］. 2021-02-19.
53	YOU C， YU X， TAN D， et al.. Gynogenesis and sex determination in large-scale loach Paramisgurnusdabryanus （Sauvage）［J］. Aquac. Int.， 2008， 16（3）：203-214.
54	CHEN S L， TIAN Y S， YANG J F， et al.. Artificial gynogenesis and sex determination in half-smooth tongue sole （Cynoglossussemilaevis）［J］. Mar. Biotechnol.， 2009， 11（2）：243-251.
55	TAO D H， LIU M Y， XIAO J Z， et al.. Advances in the research on induction means of biopolyploid ［J］. Life Sci. Res.， 2007， 11（4）：6-13.
56	刘少军，蒋励，王余德，等．一种雌、雄核发育实验设备：CN201920701195.0 ［P］. 2020-04-24.
57	FAUST E， HALVORSEN K T， ANDERSEN P， et al.. Cleaner fish escape salmon farms and hybridize with local wrasse populations ［J/OL］. Royal Soc. Open Sci.， 2018， 5（3）：171752［2021-09-28］. .
58	胡炜，汪亚平，朱作言．转基因鱼生态风险评价及其对策研究进展［J］. 中国科学C辑：生命科学， 2007，37（4）：377-381.
	HU W， WANG Y P， ZHU Z Y. Progress in the evaluation of transgenic fish for possible ecological risk and its containment strategies ［J］. Sci. Chin. Series C： Life Sci.， 2007， 37（4）：377-381.

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