Advance on Detection Methods of Nucleic Acid for Toxin-Producing Fungi in Food

doi:10.13304/j.nykjdb.2019.0920

Journal of Agricultural Science and Technology ›› 2021, Vol. 23 ›› Issue (3): 91-98.DOI: 10.13304/j.nykjdb.2019.0920

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Advance on Detection Methods of Nucleic Acid for Toxin-Producing Fungi in Food

ZHANG Qing1,2, ZHAO Xiaomei2, WANG Ping2, LIU Bing1*, CHEN Ying2*

1.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education; College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China;
2.Agro-Product Safety Research Center, Chinese Academy of Inspection and Quarantine, Beijing 100176, China

Received:2019-11-05 Online:2021-03-15 Published:2020-03-10

食品中产毒真菌核酸检测方法的研究进展

张庆1,2,赵晓美2,王娉2,刘冰1*,陈颖2*

1.天津科技大学食品科学与工程学院, 食品营养与安全国家重点实验室, 省部共建食品营养与安全重点实验室, 天津 300457;
2.中国检验检疫科学研究院农产品安全研究中心, 北京 100176

通讯作者: 陈颖 E-mail: chenyingcaiq@163.com; 刘冰 E-mail: ice6377@tust.edu.cn
作者简介:张庆 E-mail：987666458@qq.com
基金资助:
国家重点研发计划项目(2017YFC1601205)

Abstract

Abstract: The most common toxin-producing fungi in foods include Aspergillus flavus, Aspergillus ochraceus, Penicillium spp. and Fusarium spp., which can cause irreversible damage or even death to human and animal organisms. Detection of toxin-producing fungi can predict its toxin-producing performance in the early stages of toxin formation, and provide technical support for pre-treatment intervention of toxin damage. This paper summarized the conventional PCR methods, real-time fluorescent quantitative PCR method, DNA fingerprinting method and DNA barcode method for the detection of toxin-producing fungi in food, and prospected the development trend of toxin-producing fungi detection methods in the future.

Key words: toxin-producing fungi, nucleic acid, detection method

摘要： 食品中最常见的产毒真菌主要包括黄曲霉、赭曲霉、青霉属和镰刀菌属等，能够对人和动物机体造成不可逆转的伤害甚至死亡。针对产毒真菌检测可在毒素形成早期预测其产毒性能，为前置化干预毒素危害提供技术支撑，概述了近年来应用于食品中产毒真菌检测的常规PCR方法、实时荧光定量PCR方法和DNA指纹图谱方法、DNA条形码方法等，并对未来产毒真菌检测方法的发展趋势进行了展望。

关键词: 产毒真菌, 核酸, 检测方法

ZHANG Qing1,2, ZHAO Xiaomei2, WANG Ping2, LIU Bing1*, CHEN Ying2*. Advance on Detection Methods of Nucleic Acid for Toxin-Producing Fungi in Food[J]. Journal of Agricultural Science and Technology, 2021, 23(3): 91-98.

张庆1,2,赵晓美2,王娉2,刘冰1*,陈颖2*. 食品中产毒真菌核酸检测方法的研究进展[J]. 中国农业科技导报, 2021, 23(3): 91-98.

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Advance on Detection Methods of Nucleic Acid for Toxin-Producing Fungi in Food

食品中产毒真菌核酸检测方法的研究进展

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