关键词: 库尔勒, STEC, 毒力基因, 耐药性, 检测

Abstract: In order to understand the distribution of the Shiga toxin-producing Escherichia coli (STEC) and its virulence genes from sheep in Korla region of Xinjiang,  the isolation and identification of STEC on 282 samples from sheep farms, slaughterhouses and markets in Korla region of Xinjiang were carried out, and the coding of virulence genesand the drug resistance of STEC strain to 17 common antibiotics was detected. The results showed that: 72 strains of STEC were detected in mutton production of 282 samples with 25.5% of detection rate. The number of virulence genes stx1+stx2, stx1, stx2, rfbE, eae and hly in 72 STEC strains were 22, 45, 2, 0, 4, 51, respectively. Among them, there were more non-O157 STEC in the sheep farm and the detection rate was 37.3%. stx1 gene was Shiga toxin mainly and the detection rate reached 65.2%. Drug resistance was dominated from β-lactam antibiotics, aminoglycoside antibiotics and macrolide antibiotics in the 72 STEC strains. The STEC strain had severe multiple drug resistance and could be resistant to nine antibiotics. Multiple drug resistance was mainly resistance to one and two antibiotics.  This research showed that there were a large number of non-O157 STEC strains in sheep farms in the mutton production process, and the drug resistance was more serious, it was necessary to prevent source pollution.

Key words: Korla, STEC, virulence genes, drug resistance, detection