关键词: 欧洲缬草, 不定根, 诱导, 悬浮培养

Abstract: In order to obtain the ideal conditions for adventitious root induction and suspension culture of Valeriana officinalis, this paper used the leaf and stem segments of aseptic seedlings as explant to study the effects of exogenous hormone indoleacetic acid（IAA）, 1-naphthylacetic acid（NAA） and 3-indolebutyric acid （IBA） on adventitious root induction; and evaluated the effect of medium types and exogenous hormones on adventitious root suspension culture. The results showed that: stem was better than leaf to be explant, NAA was better than IBA and IAA  in the process of adventitious root induction. MS+0.5 mg·L-1 NAA was the ideal medium for inducing adventitious roots and its adventitious root induction rate was the highest, reaching 94.44%. Besides, in the process of adventitious root suspension culture, RCM was the ideal medium. Its fresh weight of harvested adventitious root could reach 7.80 g·L-1, harvested dry weight was 1.46 g·L-1 and dry matter rate was 18.73%. 0.5 mg·L-1 NAA was an ideal exogenous hormone. Its adventitious root yield could reach as high as 33.56 g·L-1 and the highest multiplication rate was 24.51. The high effective culturing system for adventitious root of Valeriana officinalis established by this study could obtain big quantity of adventitious root in short time, and provide technical basis for industrial production of adventitious roots.

Key words: Valeriana officinalis, adventitious root, induction, suspension culture