关键词: 多重表型分析, 耐盐性, 萌发期, 苗期, 高粱

Abstract: Salt tolerance in sorghum is significantly differed among various germplasms, development periods and growth conditions, therefore, establishing a comprehensive, objective and accurate method for the evaluation of salt tolerance is of great interest to screen and identify salt resistant sorghum germplasms. To this end, 64 sorghum accessions from a broad genetics background were evaluated for salt tolerance under growth chamber condition. Subsequently, 36 sorghum accessions with different degree of salt resistance were selected for field test of salt tolerance performance at seedling stage. Eventually, 13 accessions were subjected to salt tolerance evaluation back in the growth chamber at seedling stage, again. Both in the field and growth chamber, the seedling height, stem diameter and other morphological indexes along with physiological and biochemical indexes such as superoxide dismutase, proline and malondialdehyde were analyzed by subordinate function method, principal component method and cluster method. So far, two salt-tolerant varieties and two salt-sensitive varieties were stood out for subsequent studies. This study established a comprehensive salt tolerance evaluation method based on multilevel phenotypic analysis, namely, enriched morphological indexes and physiological and biochemical assay indexes were used to systematically and accurately evaluate salt tolerance in sorghum at different development stages (germination and seedling stages) and different growth conditions (field and growth chamber) with various data analysis approaches. The establishment of this method not only provided a valuable reference for the evaluation of salt tolerance in sorghum germplasm resources, but also was beneficial and instructive to the breeding research on salt tolerant sorghum cultivars.

Key words: multilevel phenotypic analysis, salt tolerance, germination, seedling stage, sorghum