关键词: 内生菌, 柑橘病害, 原生质体, 再生, 融合

Abstract: In order to integrate the excellent characteristics of biocontrol streptomyces strain ML27 and endophytic actinomycetes strain P3Y2 from citrus, the protoplast fusion between strain ML27 and P3Y2 were studied in this paper. The results showed that protoplasts with sufficient number and strong regeneration ability were obtained when ML27 and P3Y2 were cultured separately in culture medium containing 20% and 10% sucrose as well as 1.5% and 1.0% glycin, and sensitive mycelia were treated with 5 mg/mL lysozyme for 90 min and 120 min separately. The protoplasts were inactivated in 60℃ water for 10 min for ML27 and 15 min for P3Y2, or under 20W ultraviolet lamp for 7 min and 5 min.Based on the above, the protoplast fusion between P3Y2 and ML27 was executed, and one fused strain ML45 was screened preliminarily according to the cultural characteristics and the inhibition effect and the colonization ability. The experimental results were able to lay a foundation for the reform and full utilization of strain P3Y2 and ML27 with the ability of controlling the citrus diseases.

Key words: endophyte, citrus disease, protoplast, regeneration, fusion