关键词: 烟草, 根际土壤, 细菌, 宏基因组学, 冗余度分析

Abstract: In order to investigate the diversity of bacteria in tobacco rhizosphere soil, and learn the bacteria community composition and structure, this paper carried out studies and tried to provide theoretical basis and guidance for constructing a healthy tobacco soil ecosystem. Taking tobacco rhizosphere soil (squaring stage) from Liangshan prefecture of Sichuan province  in 2014 as sample, using high-throughput sequencing technology on Illumina Miseq platform, this study analyzed the soil bacteria 16S rDNA of tobacco root. The results showed that the bacterial diversity of tobacco rhizosphere soil was high, distribution structure of the main colonies among different samples were relatively stable. About phylum, there were 25 bacteria phylum detected and classified, among which Proteobacteria took clear superiority (39.04%~53.71%), followed by Bacteroidetes, Acidobacteria and Verrucomicrobia. About classification of bacteria genus, there was 576 bacteria genus detected and classified, among which Sphingomonas, Flavisolibacter and Ohtaekwangia took obvious advantages. There was a significant positive correlation between most of the major bacterial flora. The larger environmental factors influencing tobacco rhizosphere soil bacteria were soil available N,  water content and pH. The bacterial information of tobacco rhizosphere soil was obtained by the 16S rDNA sequencing technology of metagenomics. This paper  provided a new theoretical reference for studying the soil mechanism of healthy tobacco planting.

Key words: tobacco, rhizosphere soil, bacterial, metagenomics, redundancy analysis