河北省不同来源辣椒炭疽病病菌鉴定及其对苯醚甲环唑的敏感性

doi:10.13304/j.nykjdb.2025.0201

中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (10): 144-150.DOI: 10.13304/j.nykjdb.2025.0201

• 动植物健康 • 上一篇

河北省不同来源辣椒炭疽病病菌鉴定及其对苯醚甲环唑的敏感性

朱亚楠¹(), 王冰雪¹(), 侯怡娇¹, 戴祎卓¹, 裴张新², 董立坤², 胡同乐¹, 王云强³, 杨文香¹(), 张娜¹()

^1.河北农业大学植物保护学院，河北省农作物病虫害生物防治技术创新中心，河北保定 071000
^2.武汉市园林科学研究院植物保护研究所，武汉 430081
^3.保定市植保植检站，河北保定 071051

收稿日期:2025-03-19 接受日期:2025-06-06 出版日期:2025-10-15 发布日期:2025-10-15
通讯作者: 杨文香,张娜
作者简介:朱亚楠 E-mail：610953251@qq.com
王冰雪 E-mail：wangbingxue24@163.com第一联系人：朱亚楠和王冰雪为共同第一作者。
基金资助:
河北省现代农业产业技术体系建设专项(HBCT2024140207);武汉市园林和林业局科研课题和推广项目(WHGF2023A04)

Pathogen Identification of Anthracnose Disease in Chili Pepper from Hebei Province and Their Sensitivity to Difenoconazole

Yanan ZHU¹(), Bingxue WANG¹(), Yijiao HOU¹, Yizhuo DAI¹, Zhangxin PEI², Likun DONG², Tongle HU¹, Yunqiang WANG³, Wenxiang YANG¹(), Na ZHANG¹()

^1.Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province，College of Plant Protection，Hebei Agricultural University，Hebei Baoding 071000，China
^2.Institute of Plant Protection，Wuhan Academy of Landscape Sciences，Wuhan 430081，China
^3.Plant Protection and Quarantine Station of Baoding，Hebei Baoding 071051，China

Received:2025-03-19 Accepted:2025-06-06 Online:2025-10-15 Published:2025-10-15
Contact: Wenxiang YANG,Na ZHANG

摘要/Abstract

摘要：

为明确河北省鸡泽县和广宗县辣椒炭疽病病原，以田间采集的辣椒炭疽病病果为材料，利用组织分离法获得分离物71株，其中鸡泽县32株，广宗县39株。通过形态学和分子生物学鉴定，确定鸡泽县的32株中有24株为斯高维尔炭疽菌（Colletotrichum scovillei），另8株为平头炭疽菌（C. truncatum），分离比例分别为75.00%和25.00%；广宗县的39株中有32株为斯高维尔炭疽菌，另7株为平头炭疽菌，分离比例分别为82.05%和17.95%。采用菌丝生长速率法测定了46株斯高维尔炭疽菌对苯醚甲环唑的敏感性，结果表明，苯醚甲环唑对这些菌株的半数效应浓度（median effect concentration，EC₅₀）为0.143 0~0.434 8 μg·mL^-1，平均0.289 8 μg·mL^-1。研究结果明确了河北省南部辣椒炭疽病的病原菌，为河北省辣椒生产中防治由斯高维尔炭疽菌引起的炭疽病提供理论依据和用药参考。

关键词: 辣椒炭疽病, 病原鉴定, 苯醚甲环唑, 药剂敏感性, 斯高威尔炭疽菌

Abstract:

To identify the pathogen of anthracnose on Capsicum annuum L. in Jize and Guangzong county in Hebei province， diseased pepper fruits were collected. 71 strains were isolated， in which 32 strains from Jize county， and 39 from Guangzong county. By morphological and molecular identification， 24 strains were identified as Colletotrichum scovillei and 8 strains were as C. truncatum in 32 strains from Jize county， acounting for 75.00% and 25.00%， respectively， 32 strains were identified as Colletotrichum scovillei and 7 strains were as C. truncatum in 39 strains from Guangzong county， acounting for 82.05% and 17.95%， respectively. Fungicide difenoconazole was employed to assay the sensitivity of 46 tested strains， the median effect concentration （EC₅₀） were ranging from 0.143 0 to 0.434 8 μg·mL^-1， with average of 0.289 8 μg·mL^-1. Above results identified the pathogen of pepper anthracnose in south of Hebei province， and provided theoretical basis and fungicide recommendation for the prevention and control of pepper anthracnose caused by C. scovillei.

Key words: chili anthracnose disease, pathogen identification, difenoconazole, sensitivity to fungicide, Colletotrichum scovillei

中图分类号:

S432

朱亚楠, 王冰雪, 侯怡娇, 戴祎卓, 裴张新, 董立坤, 胡同乐, 王云强, 杨文香, 张娜. 河北省不同来源辣椒炭疽病病菌鉴定及其对苯醚甲环唑的敏感性[J]. 中国农业科技导报, 2025, 27(10): 144-150.

Yanan ZHU, Bingxue WANG, Yijiao HOU, Yizhuo DAI, Zhangxin PEI, Likun DONG, Tongle HU, Yunqiang WANG, Wenxiang YANG, Na ZHANG. Pathogen Identification of Anthracnose Disease in Chili Pepper from Hebei Province and Their Sensitivity to Difenoconazole[J]. Journal of Agricultural Science and Technology, 2025, 27(10): 144-150.

图/表 5

表1 试验用于对炭疽病菌进行多基因联合鉴定的引物

Table 1 Primers used in this research for identification of the Colletotrichum spp.

名称 Name	序列 Sequence （5’-3’）	参考文献 Reference
ITS1	TCCGTAGGTGAACCTGCGG	［18］
ITS4	TCCTCCGCTTATTATATGC	［18］
GDF1	GCCGTCAACGACCCCTTCATTGA	［19］
GDR1	GGGTGGAGTCGTACTTGAGCATGT	［19］
ACT-512F	ATGTGCAAGGCCGGTTTCGC	［20］
ACT-783R	TACGAGTCCTTCTGGCCCAT	［20］
CHS-79F	TGGGGCAAGGATGCTTGGAAGAAG	［20］
CHS-345R	TGGAAGAACCATCTGTGAGAGTTG	［20］

图1 GZ6和JZ15-1菌落、分生孢子和附着胞显微观察A：GZ6菌落的正面和背面形态；B：GZ6分生孢子；C：GZ6菌丝附着胞；D：JZ15-1菌落的正面和背面形态；E：JZ15-1分生孢子；F：JZ15-1菌丝附着胞；标尺=10 μm

Fig. 1 Microscopic observation of GZ6 and JZ15-1 colonies， conidia and appressoriumA： Front and back morphology of GZ6 colon； B： Conidia of GZ6； C： Mycelial appressoria of GZ6； D： Front and back morphology of JZ15-1 colon； E： Conidia of JZ15-1； F： Mycelial appressoria of JZ15-1； Bar=10 μm

图2 GZ6和JZ15-1分离前和接种后的病症A：GZ6分离前；B：GZ6接种后；C：JZ15-1分离前；D：JZ15-1接种后

Fig. 2 Symptom before separation and inoculation of GZ6 and JZ15-1A： Symptom before separation of GZ6； B： Symptom inoculated GZ6； C： Symptom before separation of JZ15-1； D： Symptom inoculated JZ15-1

图3 炭疽菌的系统发育树

Fig. 3 Phylogenetic tree of Colletotrichum

图4 46株斯高威尔炭疽菌对苯醚甲环唑的EC50

Fig. 4 EC50 of 46 C. scovillei strains to difenoconazole

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河北省不同来源辣椒炭疽病病菌鉴定及其对苯醚甲环唑的敏感性

Pathogen Identification of Anthracnose Disease in Chili Pepper from Hebei Province and Their Sensitivity to Difenoconazole

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