关键词: 百里香酚, 秀丽线虫, 生物防治

Abstract: Synthetic pesticides used to control plant parasitic nematodes have seriously polluted the ecological environment. Therefore it is urgent to develop natural biological pesticides. All nematode species are similar, and the difference between parasitic nematodes and model animal Caenorhabditis elegansis are less than the difference between species of parasitic nematodes. Thus the present study investigated the effect of thymol, a natural product from Thymus mongolicus Ronn., on C. elegans  at the stages of growth and development (L4 stage) and reproductive (egg stage). The lifespan, locomotion capacity and survival sub-algebraic phenotype of C. elegansis were examined. The results showed that the median lethal concentration and minimum lethal concentration was 50 mg/L and 125 mg/L, respectively, when the C. elegans at L4 stage was treated with thymol for 4 h. When C. elegans at egg stage was treated with thymol for 4 h, the median lethal concentration and minimum lethal concentration was 50 mg/L and 100 mg/L, respectively. Meanwhile thymol significantly reduced the athletic ability of C. elegans at L4 and egg stages, and the surviving progeny number of C. elegans at egg stage. Sum up, the treatment with 125 mg/L thymol on nematodes for 4 h had the best insecticidal effect and could control plant parasitic nematodes with low cost,  high efficiency and environmental friendly.

Key words: thymol, Caenorhabditis elegans, biological control