关键词: 魁蚶, 牛磺酸, 提取

Abstract: With the development of Scapharca broughtonii breeding scale, a large number of viscera byproducts were produced. In order to comprehensively utilize these byproducts, this study took Scapharca broughtonii as raw material, confirmed the taurine extraction process condition by ethanol refluxing through single factor and orthogonal test，and measured its content by high performance liquid chromatography (HPLC). The optimal extraction conditions were: solid-liquid ratio 1 g∶12 mL, ethanol concentration 75%, extraction time 50 min, temperature 70℃, the extraction amount 5.36 mg/g. The taurine was in the range of 5.0~20.0 μg/mL with good linear correlation, the correlation coefficient R2=0.999 7, the recoveries were 89.27%~97.27% and the RSD was 1.83%, which indicated that HPLC had determined the taurine content in aquatic products with high accuracy and good reproducibility. The taurine extraction content was 6.29 mg/g, which was the highest in viscera of Scapharca broughtonii, foot took second place, blood was the lowest. These results suggested that the taurine content in Scapharca broughtonii was rich and varied in different tissues. This study opened up a new way for the development and utilization of Scapharca broughtonii.

Key words: Scapharca broughtonii, taurine, extraction