关键词: 棉花, WRKY, 鉴定, 进化分析

Abstract: The WRKY transcription factor family is widely involved in regulating plant development and defense response against various biotic and abiotic stresses. In this study, the gene number, classification, conserved motif of the WRKY transcriptional factors in G. arboretum were analyzed. A total of 109 GaWRKYs were identified in G. arboretum. The coding sequences were 474~3 528 bp in length，and the genes were distributed in 13 different chromosomes. According to the structural feature of WRKY domain and evolution of the protein family, the 109 GaWRKYs were classified into 3 groups (G1, G2 and G3). G1 included 21 WRKY members, among which 18 members had 2 WRKY domains; G2 included 73 WRKY members, and they were divided into 5 subgroups (G2-a, G2-b, G2-c, G2-d and G2-e); G3 had 15 WRKY members. The WRKY transcription factors showed similar motif compositions within each group, but there were variants of WRKYGQK peptide and zinc finger motif. However, the amino acid conserved domains of WRKY members were different among different groups. Phylogenetic trees of the WRKY gene of Arabidopsis thaliana and cotton showed that functional similar genes got together. It indicated that those GaWRKYs together with Arabidopsis thaliana anti reverse function gene were likely involved in plant responses to various stresses. The present results provided theoretical basis for further discovery and mining functional WRKY gene in cotton.

Key words: cotton, WRKY, identification, evolution analysis