关键词: 烟草绿原酸, 脂质体, 制备工艺, 稳定性

Abstract: This paper screened 3 preparation methods of tobacco chlorogenic acid (CA) nanoliposome, and analyzed their stability. Dialysis method was employed to determine entrapment efficiency (EE) of chlorogenic acid liposomes. Thin film hydration, reverse phase evaporation technique, and ethyl alcohol injection methods were screened based on EE and diameter of liposomes. The effects of film (lecithin+cholesterol)/CA mass ratio, lecithin/cholesterol mass ratio and ultrasonication time on EE and effective loading ability of liposomes were investigated. Orthogonal combination design was used to determine the best process parameters. Finally, the stability of the prepared liposomes was evaluated. The results showed that when film/CA mass ratio was 9∶1, lecithin/cholesterol mass ratio was 4∶1 and ultrasonication time was 5 min, the CA nanoliposomes showed regular spherical. Its average grain diameter was 77.16 nm with good dispersibility. The embedding rate and efficient loading capacity could be up to 88.02% and 9.69%, respectively. Post-embedding liposome thermal and alkaline stabilities were all improved. UV test showed that CA was encapsulated successfully into nanoliposomes. Above results showed that CA nanoliposomes prepared by ethyl alcohol injection method had small grain diameter, good dispersion, better thermal stability, alkaline stability and storage stability.

Key words: tobacco chlorogenic acid, lipidosome, preparation technics, stability