Research Progress on Application of Bacillus velezensis in Animal Husbandry

doi:10.13304/j.nykjdb.2025.0310

Abstract

Abstract:

Bacillus velezensis， as a new type of probiotic， is widely used in fields such as industrial fermentation and agricultural production. The functions of active substances produced by Bacillus velezensis， such as enzyme production and disease resistance etc， were introduced in detail. Its latest application status in livestock and aquaculture were reviewed. In view of the current problems existing in Bacillus velezensis， the future research focuses and development directions were prospected， which provided references for the in-depth research and practical application of Bacillus velezensis.

Key words: Bacillus velezensis, aquaculture, livestock, growth performance, immunity

摘要：

贝莱斯芽孢杆菌（Bacillus velezensis）作为新型的益生菌在工业发酵、农业生产等领域应用广泛。详细介绍了贝莱斯芽孢杆菌产活性物质的功能，产酶和抗病等；综述了其在畜禽养殖和水产养殖的最新应用现状；并针对贝莱斯芽孢杆菌目前存在的问题，展望了未来的研究重点和发展方向，为贝莱斯芽孢杆菌的深入研究以及实际应用提供参考。

关键词: 贝莱斯芽孢杆菌, 水产养殖, 禽畜养殖, 生长性能, 免疫力

CLC Number:

Wenhao ZHOU, Yuhan ZHANG, Delong MENG, Hui LIANG, Yuanpei ZHANG, Yalin YANG, Zhen ZHANG, Yuanyuan YAO, Chao RAN, Zhigang ZHOU. Research Progress on Application of Bacillus velezensis in Animal Husbandry[J]. Journal of Agricultural Science and Technology, 2025, 27(11): 1-7.

周文豪, 张玉涵, 孟德龙, 梁慧, 张元培, 杨雅麟, 张震, 药园园, 冉超, 周志刚. 贝莱斯芽孢杆菌在养殖业中的应用研究进展[J]. 中国农业科技导报, 2025, 27(11): 1-7.

References 49

[1]	SURESH G, DAS R K, KAUR BRAR S, et al.. Alternatives to antibiotics in poultry feed: molecular perspectives [J]. Crit. Rev. Microbiol., 2018, 44(3): 318-335.
[2]	SANYAL D, SHIVRAM A, PANDEY D, et al.. Mapping dihydropteroate synthase evolvability through identification of a novel evolutionarily critical substructure [J/OL]. Int. J. Biol.Macromol., 2025,311: 143325 [2025-03-20]. .
[3]	NADELLA R K, PANDA S K, BADIREDDY M R, et al.. Multi-drug resistance, integron and transposon-mediated gene transfer in heterotrophic bacteria from Penaeus vannamei and its culture environment [J]. Environ. Sci. Pollut. Res. Int., 2022, 29(25): 37527-37542.
[4]	VERMA J, DEVI S, NARANG A, et al.. Probiotic potential of Streptomyces levis strain HFM-2 isolated from human gut and its antibiofilm properties against pathogenic bacteria [J/OL].BMC Microbiol., 2024, 24(1): 208 [2025-03-20]. .
[5]	AMIIN M K, LAHAY A F, PUTRIANI R B, et al.. The role of probiotics in vannamei shrimp aquaculture performance-a review [J]. Vet. World, 2023, 16(3): 638-649.
[6]	ZHU C Y, LUO Y Q, XU K J, et al.. Effects of dietary supplementation with Bacillus subtilis and bacteriophage on growth performance,intestinal morphology and microbiota structure in 0-90 d MaGang geese [J/OL]. Front. Nutr., 2025,12: 1537724 [2025-03-20]. .
[7]	KOIKE S, UENO M, ASHIDA N, et al.. Effect of Bacillus subtilis C-3102 supplementation in milk replacer on growth and rumen microbiota in preweaned calves [J/OL]. Anim. Sci. J., 2021, 92(1): e13580 [2025-03-20]..
[8]	TANG Y Y, LI T, HUANG Y H, et al.. Comprehensive phenotypic characterization and genomic analysis unveil the probiotic potential of Bacillus velezensis K12 [J/OL]. Animals, 2025, 15(6): 798 [2025-03-20]. .
[9]	DUNLAP C A, BOWMAN M J, SCHISLER D A, et al.. Genome analysis shows Bacillus axarquiensis is not a later heterotypic synonym of Bacillus mojavensis;reclassification of Bacillus malacitensis and Brevibacterium halotolerans as heterotypic synonyms of Bacillus axarquiensis [J]. Int. J. Syst.Evol. Microbiol., 2016, 66(6): 2438-2443.
[10]	ZHANG J X, WU J Y, CHEN Y Y, et al.. Complete genome analysis of Bacillus velezensis HF-14, 109 with potential for broad-spectrum antimicrobial activity and high enzyme-producing ability from common carp (Cyprinus carpio L.) [J/OL]. Mol.Genet. Genomics, 2025, 300(1): 26 [2025-03-20]. .
[11]	HEO S J, KIM A J, PARK M J, et al.. Nutritional and functional properties of fermented mixed grains by solid-state fermentation with Bacillus amyloliquefaciens 245 [J/OL]. Foods,2020, 9(11): 1693 [2025-03-20]. .
[12]	WANG Z M, WANG S, BAI H, et al.. Characterization and application of Bacillus velezensis D6 co-producing α-amylase and protease [J]. J. Sci. Food Agric., 2024, 104(15): 9617-9629.
[13]	WANG J, XIE X Y, LI B, et al.. Complete genome analysis and antimicrobial mechanism of Bacillus velezensis GX0002980 reveals its biocontrol potential against mango anthracnose disease [J/OL]. Microbiol. Spectr., 2025, 13(6): e0268524 [2025-03-20]. .
[14]	HAN D M, BAEK J H, CHUN B H, et al.. Fermentative features of Bacillus velezensis and Leuconostoc mesenteroides in doenjang-meju,a Korean traditional fermented soybean brick [J]. Food Microbiol., 2023, 110: 104186-104198.
[15]	TRIPATHI V, GAUR V K, THAKUR R S, et al.. Assessing the half-life and degradation kinetics of aliphatic and aromatic hydrocarbons by bacteria isolated from crude oil contaminated soil [J/OL]. Chemosphere, 2023, 337: 139264 [2025-03-20]..
[16]	BISHOP G, STYLES D, LENS P N L. Recycling of European plastic is a pathway for plastic debris in the ocean [J/OL].Environ. Int., 2020, 142: 105893 [2025-03-20]. .
[17]	LIU X R, ZHANG Y M, SUN Q F, et al.. Rapid colonization and biodegradation of untreated commercial polyethylene wrap by a new strain of Bacillus velezensis C5 [J/OL]. J. Environ.Manage., 2022, 301:113848 [2025-03-20]. .
[18]	HUANG L, MENG D, TIAN Q P, et al.. Characterization of a novel carboxylesterase from Bacillus velezensis SYBC H47 and its application in degradation of phthalate esters [J]. J. Biosci.Bioeng., 2020, 129(5): 588-594.
[19]	JIANG N, WANG T J, FANG Y, et al.. A novel protein demonstrating antibacterial activity against multidrug-resistant Escherichia coli purified from Bacillus velezensis CB6 [J]. Foods,2025, 14(7): 1255-1268.
[20]	CHEN J L, FENG Y Z, MA J C, et al.. Genomic and metabolomic insights into the antimicrobial compounds and plant growth-promoting potential of Bacillus velezensis B115 [J].Sci. Rep., 2025, 15: 10666-10678.
[21]	ZHAO Q, ALI Q, YUAN W W, et al.. Role of iturin from Bacillus velezensis DMW1 in suppressing growth and pathogenicity of Plectosphaerella cucumerina in tomato by reshaping the rhizosphere microbial communities [J/OL].Microbiol. Res., 2025, 296: 128150 [2025-03-20]. .
[22]	YU F T, SHEN Y Y, CHEN S L, et al.. Analysis of the genomic sequences and metabolites of Bacillus velezensis YA215 [J].Biochem. Genet., 2024, 62(6): 5073-5091.
[23]	EMAM A M, DUNLAP C A. Genomic and phenotypic characterization of Bacillus velezensis AMB-y1; a potential probiotic to control pathogens in aquaculture [J]. Antonie Van Leeuwenhoek, 2020, 113(12): 2041-2052.
[24]	PUAN S L, ERRIAH P, YAHAYA N M, et al.. Genome-guided identification and characterisation of broad-spectrum antimicrobial compounds of Bacillus velezensis strain PD9 isolated from stingless bee Propolis [J/OL]. Probiotics Antimicrob. Proteins, 2025, 25: 10451-3 [2025-03-20]. .
[25]	HU H, WU C Y, GE F L, et al.. Poly-γ-glutamic acid-producing Bacillus velezensis fermentation can improve the feed properties of soybean meal [J/OL]. Food Biosci., 2023, 53:102503 [2025-03-20]. .
[26]	ZENG J R, HUANG W Q, TIAN X F, et al.. Brewer’s spent grain fermentation improves its soluble sugar and protein as well as enzymatic activities using Bacillus velezensis [J]. Process. Biochem., 2021, 111: 12-20.
[27]	LIU Y, XIONG M Q, HU X, et al.. Dietary Bacillus velezensis KNF-209 supplementation improves growth performance,enhances immunity,and promotes gut health in broilers [J].Poult. Sci., 2024, 103(9): 103946-103958.
[28]	LI C, LI S Z, DANG G Q, et al.. Screening and characterization of Bacillus velezensis LB-Y-1 toward selection as a potential probiotic for poultry with multi-enzyme production property [J/OL]. Front. Microbiol., 2023, 14: 1143265 [2025-03-20]. .
[29]	QUACH N T, VU T H N, NGUYEN N A, et al.. Phenotypic features and analysis of genes supporting probiotic action unravel underlying perspectives of Bacillus velezensis VTX9 as a potential feed additive for swine [J/OL]. Ann. Microbiol.,2021, 71(1): 36 [2025-03-20]. .
[30]	LI Y Q, LI X, JIA D, et al.. Complete genome sequence and antimicrobial activity of Bacillus velezensis J T3-1, a microbial germicide isolated from yak feces [J/OL]. 3 Biotech, 2020, 10(5): 231 [2025-03-20]. .
[31]	JI L B, SHEN J K, LIU C C, et al.. Dietary Bacillus velezensis improves piglet intestinal health and antioxidant capacity via regulating the gut microbiota [J]. Int. J. Mol. Sci., 2025, 26(12):5875-5889.
[32]	AFROJ S, BRANNEN A D, NASRIN S, et al.. Bacillus velezensis AP183 inhibits Staphylococcus aureus biofilm formation and proliferation in murine and bovine disease models [J/OL]. Front.Microbiol., 2021, 12: 746410 [2025-03-20]. .
[33]	TSAI C Y, HU S Y, SANTOS H M, et al.. Probiotic supplementation containing Bacillus velezensis enhances expression of immune regulatory genes against pigeon circovirus in pigeons (Columba livia) [J]. J. Appl. Microbiol., 2021, 130(5): 1695-1704.
[34]	ZHENG J, ZENG H, ZHANG Q, et al.. Effects of intranasal administration with a symbiotic strain of Bacillus velezensis NSV2 on nasal cavity mucosal barrier in lambs [J/OL]. Vet. Res.Commun., 2024, 49(1): 21 [2025-03-20]..
[35]	GAO X Y, LIU Y, MIAO L L, et al.. Characterization and mechanism of anti-Aeromonas salmonicida activity of a marine probiotic strain,Bacillus velezensis V4 [J]. Appl. Microbiol.Biotechnol., 2017, 101(9): 3759-3768.
[36]	THURLOW C M, WILLIAMS M A, CARRIAS A, et al.. Bacillus velezensis AP193 exerts probiotic effects in channel catfish (Ictalurus punctatus) and reduces aquaculture pond eutrophication [J]. Aquaculture, 2019, 503: 347-356.
[37]	YANG H W, DU D D, ZHANG Q S, et al.. Dietary Bacillus velezensis T23 fermented products supplementation improves growth,hepatopancreas and intestine health of Litopenaeus vannamei [J/OL]. Fish Shellfish. Immunol., 2024, 149: 109595 [2025-03-20]. .
[38]	ABDELSAMAD A E M, SAID R E M, ASSAS M, et al.. Effects of dietary supplementation with Bacillus velezensis on the growth performance, compositionbody, antioxidant, immune-related gene expression,and histology of Pacific white shrimp,Litopenaeus vannamei [J/OL]. BMC Vet. Res., 2024, 20(1): 368 [2025-03-20]. .
[39]	JI Z H, LU X, XUE M Y, et al.. The probiotic effects of host-associated Bacillus velezensis in diets for hybrid yellow catfish (Pelteobagrus fulvidraco ♀ × Pelteobagrus vachelli ♂) [J]. Anim.Nutr., 2023, 15: 114-125.
[40]	TAO B Y, ZHANG C X, LI X, et al.. Postbiotics of Bacillus subtilis LCBS1 have beneficial effects on bullfrogs (Lithobates catesbeianus) [J/OL]. Aquaculture, 2023, 574: 739699 [2025-03-20]. .
[41]	MONZÓN-ATIENZA L, BRAVO J, TORRECILLAS S, et al..Isolation and characterization of a Bacillus velezensis D-18 strain, as a potential probiotic in European seabass aquaculture [J]. Probiotics Antimicrob. Proteins, 2021, 13(5): 1404-1412.
[42]	YI Y L, ZHANG Z H, ZHAO F, et al.. Probiotic potential of Bacillus velezensis JW: antimicrobial activity against fish pathogenic bacteria and immune enhancement effects on Carassius auratus [J]. Fish Shellfish. Immunol., 2018, 78: 322-330.
[43]	CAO L N, PAN L F, GONG L, et al.. Interaction of a novel Bacillus velezensis (BvL03) against Aeromonas hydrophila in vitro and in vivo in grass carp [J]. Appl.Microbiol. Biotechnol.,2019, 103(21/22): 8987-8999.
[44]	ZHANG D X, KANG Y H, ZHAN S, et al.. Effect of Bacillus velezensis on Aeromonas veronii-Induced intestinal mucosal barrier function damage and inflammation in crucian carp (Carassius auratus) [J/OL]. Front.Microbiol., 2019, 10: 2663 [2025-03-20]. .
[45]	SAM-ON M F S, MUSTAFA S, YUSOF M T, et al.. Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing vibriosis and aeromonosis [J/OL]. Microb. Pathog., 2022, 164:105417 [2025-03-20]. .
[46]	WU Z B, QI X Z, QU S Y, et al.. Dietary supplementation of Bacillus velezensis B8 enhances immune response and resistance against Aeromonas veronii in grass carp [J]. Fish Shellfish. Immunol., 2021, 115: 14-21.
[47]	ZHANG D F, GAO Y X, KE X L, et al.. Bacillus velezensis LF01: in vitro antimicrobial activity against fish pathogens,growth performance enhancement, and disease resistance against streptococcosis in Nile tilapia (Oreochromis niloticus) [J]. Appl. Microbiol. Biotechnol., 2019, 103(21/22): 9023-9035.
[48]	ZHAO C Y, MEN X H, DANG Y J, et al.. Probiotics mediate intestinal microbiome and microbiota-derived metabolites regulating the growth and immunity of rainbow trout (Oncorhynchus mykiss) [J/OL]. Microbiol. Spectr., 2023, 11(2):e03980-22 [2025-03-20]. .
[49]	AMOAH K, TAN B P, ZHANG S, et al.. Host gut-derived Bacillus probiotics supplementation improves growth performance,serum and liver immunity,gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) [J]. Anim. Nutr., 2023, 14: 163-184.