Abstract: In order to investigate the depositions of several mineral elements and the mRNA expression levels of mineral-related genes in different tissues of Runzhou white crested duck, the contents of Mg, K, Fe, Zn and Se in breast muscle, thigh muscle, liver, skin and tibia at 0, 21, 35, 49 and 63 days of age were measured by atomic fluorescence spectrophotometer. The mRNA expression levels of mineral-related genes were detected by qRT-PCR. The results revealed that the depositions of Mg and K were basically the same in all tissues, showing a significant positive correlation (P<0.05). In  the breast muscle, thigh muscle and liver, the contents of almost all the mineral elements reached the peak value (P<0.05) at 49—63 days of age. The expression levels of Mg-related genes TRPM6 and TRPM7 were the highest at 21 and 49 days of age, respectively (P<0.05); the expression levels of K-related genes ATP1A1 and ATP1B1 were the highest at 49 days of age (P<0.05); the expression of Fe-related gene FTH1 significantly increased (P<0.05) with the change of age, which had the greatest expression at the period of 49—63 days of age (P<0.05); the expression levels of Zn-related genes ATP6 and ATP8 were the highest at 49 and 35 days of age, respectively (P<0.05); the expression levels of Se-related genes GPX1 and GPX4 were the highest at 63 days of age (P<0.05). In addition, there was a significant positive correlation between the expression level of TRPM7 and the deposition of Mg in liver (r=0.942, P<0.05). Mg and K could be used as joint indicators for the precise breeding of high quality strain of Runzhou white crested duck; 49—63 days of age could be used as the reference of the best marketing age; TRPM7 gene could be used as the key gene for the detection of Mg. The results of this study provided a theoretical basis for breeding index and breeding efficiency of small-sized high quality meat duck.

Key words: Runzhou white crested duck, mineral elements, gene expression, correlation

摘要： 为了探讨润州凤头白鸭不同组织中主要矿物元素的沉积规律及相关基因的mRNA表达水平，利用原子荧光光度计分别测定了0、21、35、49和63日龄润州凤头白鸭胸肌、腿肌、肝脏、背皮和胫骨组织中的镁、钾、铁、锌和硒等矿物元素含量，采用qRT-PCR法分析不同日龄肝脏组织中各矿物元素相关基因表达水平及其与矿物元素沉积之间的关系。结果表明：镁与钾元素的沉积规律在所有组织中基本一致，且两者呈显著性正相关（P<0.05），在胸肌、腿肌和肝脏组织中，几乎所有矿物元素的含量在49—63日龄达到峰值（P<0.05）并趋于稳定。镁元素相关基因TRPM6和TRPM7分别在21和49日龄时的表达水平最高（P<0.05）；钾元素相关基因ATP1A1和ATP1B1均在49日龄时的表达水平最高（P<0.05）；铁元素相关基因FTH1的表达规律表现为随日龄增加呈显著性（P<0.05）升高的趋势，在49—63日龄时的表达水平最高（P<0.05）；锌元素相关基因ATP6和ATP8分别在49和35日龄时的表达水平最高（P<0.05）；硒元素相关基因GPX1和GPX4均在63日龄时表达水平最高（P<0.05）。此外，镁元素相关基因TRPM7的表达水平与镁元素在肝脏组织中的沉积呈显著性正相关（r=0.942，P<0.05），其他基因表达水平与矿物元素沉积存在一定的正负相关性，但不显著（P>0.05）。镁元素和钾元素可作为联合指标用于润州凤头白鸭优质品系的精准选育；49—63日龄可作为优质润州凤头白鸭最佳出栏日龄的参考日龄；TRPM7基因可作为镁元素含量检测关键标志基因。上述结果为小体型优质肉鸭的选育指标和选育效率提供理论依据。

关键词: 润州凤头白鸭, 矿物元素, 基因表达, 相关性