Abstract: In order to understand the current situation of resistance of Botrytis cinerea in eastern Helan mountain to 8 kinds of fungicides used in production, the spore germination method was used to detect the resistance of Botrytis cinerea on the main grape varieties, such as Cabernet Sauvignon, Chardonnay,  Red Globe, Zana and Daqing, in Ningxia state farm, Qingtongxia city and along the mountain of Helan. The results showed that: ① from the level of fungicide, the EC50 value of the tested strains was 0.184 6 μg·mL-1, with the strongest sensitivity to fluazinam; the EC50 value of the tested strains to boscalid and fludioxonil was lower than 49.205 7 μg·mL-1; and the strains of Botrytis cinerea showed the strongest resistance to carbendazim and SYP-Z048, with EC50 values up to 36 760.563 6 and 248.055 4 μg·mL-1, respectively. ② from different areas, the spore germination inhibition rate of Botrytis cinerea in Qingtongxia was 61.29% on average, which was sensitive to fungicides; the next was along the Helan Mountain, which was 60.76% on average; the average of Ningxia Agricultural Reclamation was 56.35%. ③ from the perspective of different varieties, the fresh grape varieties were generally better, and the spore germination inhibition rate of Botrytis cinerea was 71.40% on average, which was more sensitive to fungicides; while the spore germination inhibition rate of Botrytis cinerea on wine grape varieties was 4688% on average, which was more resistant to fungicides. In different production areas, the different resistant fungicides could be used alternately according to the occurrence of diseases, meteorological conditions and environmental factors. And concurrently the concentration of fungicides could be adjusted appropriately to achieve the purpose of high-efficiency utilization and delay the emergence of resistance of pathogens.

Key words: eastern Helan mountain, Botrytis cinerea, fungicides, drug resistance

摘要： 为了解贺兰山东麓葡萄灰霉病菌对目前生产上所使用的8大类杀菌剂的抗药性现状，采用孢子萌发测定法分别对宁夏农垦、青铜峡市、贺兰山沿山等葡萄主栽区的赤霞珠、霞多丽、红地球、乍娜、大青等主栽葡萄品种上的灰霉病菌进行抗药性检测。结果表明：①从药剂水平看，供试灰霉病菌株对氟啶胺的有效抑制中浓度（EC50）均为0.184 6 μg·mL-1，敏感性最强；其次为啶酰菌胺和咯菌腈，其EC50均低于49.205 7 μg·mL-1；而供试灰霉病菌株对多菌灵和啶菌噁唑产生的抗药性最强，其EC50分别高达36 760.563 6和248055 4 μg·mL-1。②从不同地区看，青铜峡地区的灰霉病菌孢子萌发抑制率平均值为61.29%，对药剂敏感性较好；其次是贺兰山沿山，灰霉病菌孢子萌发抑制率平均值为60.76%；宁夏农垦灰霉病菌孢子萌发抑制率平均值为56.35%。③从不同品种看，鲜食葡萄品种整体较好，其灰霉病菌孢子萌发抑制率平均值为71.40%，对药剂敏感性较好；而酿酒葡萄品种灰霉病菌孢子萌发抑制率平均值为46.88%，抗药性较强。各产区可依据病害发生情况、气象条件及环境因素交替使用不同抗药性杀菌剂，并适当调整药剂浓度，达到高效利用和延缓病菌产生抗药性的目的。

关键词: 贺兰山东麓, 葡萄灰霉病菌, 杀菌剂, 抗药性