FeNPs对苗期棉花根系生长及其对干旱响应的影响

doi:10.13304/j.nykjdb.2023.0625

摘要/Abstract

摘要：

纳米铁（FeNPs）作为一种新型纳米金属肥料，其在棉花生长发育和抗逆调节中的研究很少。设置4个FeNPs处理水平（0、1.0、2.5、5.0 mg·L^-1）研究其对水培棉苗根系生长和干旱胁迫（PEG）下生理指标的影响。结果发现，5.0 mg·L^-1 FeNPs培养的棉苗总根系长度、总根表面积、投影面积、根尖数目及根、茎、叶的鲜重和干重均显著高于对照和1.0、2.5 mg·L^-1 FeNPs。棉花在0和5.0 mg·L^-1 FeNPs的Hogland营养液中培养至四叶期，分别施加10%（质量体积分数）PEG-6000，结果发现，无FeNPs处理较5.0 mg·L^-1 FeNPs处理的植株更萎蔫，其根、茎、叶鲜重显著小于FeNPs处理棉苗；在胁迫6 h内，FeNPs促进棉叶SOD、POD和CAT活性显著升高，MDA含量显著下降；而9 h后，SOD和CAT活性出现大幅下降、MDA含量显著上升。综上所述，适当水平FeNPs有助于棉苗根系生长、干物质积累和抗氧化酶活性增强，进而增强棉苗抗旱性。

关键词: 棉花, FeNPs, 根系, 生理指标, 抗逆性

Abstract:

Nano iron （FeNPs） is a new type of nano metal fertilizer， but its regulation effects on cotton growth and stress resistance are rarely studied. 4 FeNPs treatment levels （0， 1.0， 2.5， 5.0 mg·L^-1） were set to study its effect on root morphology and physiological indexes of hydroponic cotton seedlings under PEG stress. The results showed that total root length， total root surface area， projected area， number of root tips and fresh and dry weight of roots， stems and leaves cultured with 5.0 mg·L^-1 FeNPs were significantly higher than those cultured with 1.0 and 2.5 mg·L^-1 FeNPs. Cotton seedlings were cultured in Hogland nutrient solution with 0 and 5.0 mg·L^-1 FeNPs， and then 10% PEG-6000 was applied at the four-leaf stage. The results showed that the cotton seedlings without FeNPs were more wilting than those treated with 5.0 mg·L^-1 FeNPs， and the fresh weight of root， stem and leaf was significantly lower than that with FeNPs. In addition， FeNPs significantly increased SOD， POD and CAT activities and significantly decreased MDA content in cotton leaves within 6 h of stress. After 9 h， SOD and CAT activities decreased and MDA content increased significantly. In summary， appropriate level of FeNPs would be beneficial to root growth， dry matter accumulation and antioxidant enzyme activity of cotton seedlings， thus enhancing the drought resistance of cotton seedlings.

Key words: cotton, FeNPs, roots, physiological indexes, stress resistance

中图分类号:

S562

陈炟, 巨吉生, 马麒, 徐守振, 刘娟娟, 袁文敏, 李吉莲, 王彩香, 宿俊吉. FeNPs对苗期棉花根系生长及其对干旱响应的影响[J]. 中国农业科技导报, 2023, 25(11): 49-57.

Da CHEN, Jisheng JU, Qi MA, Shouzhen XU, Juanjuan LIU, Wenmin YUAN, Jilian LI, Caixiang WANG, Junji SU. Effects of FeNPs on Cotton Roots Growth and Its Response to Drought Stress at Seedling Stage[J]. Journal of Agricultural Science and Technology, 2023, 25(11): 49-57.

图/表 6

图1 不同FeNPs水平对棉花根系相关指标的影响注：图中不同小写字母表示差异显著（P<0.05）。

Fig. 1 Effects of different FeNPs levels on root related indexes of cotton.Note：Different letters in the figure mean significant difference at P<0.05 level.

图2 不同FeNPs水平下陆地棉苗期根系形态

Fig. 2 Root morphology under different levels of FeNPs at seedling stage

图3 不同FeNPs水平对棉花株高、主根长度及根、茎、叶干物质积累的影响注：图中不同小写字母表示差异显著（P<0.05）。

Fig. 3 Effects of different FeNPs levels on plant height， main root length and dry matter accumulation in roots， stems and leaves of cottonNote：Different letters in the figure mean significant difference at P<0.05 level.

图4 10% PEG胁迫条件下FeNPs对棉花根、茎、叶生物量的影响注：图中不同小写字母表示差异显著（P<0.05）。

Fig. 4 Effects of the FeNPs on root， stem and leaf biomass of cotton under 10% PEG stress.Note：Different letters in the figure mean significant difference at P<0.05 level.

图5 10% PEG胁迫条件下FeNPs影响的棉花苗期植株形态

Fig. 5 Plant morphology of cotton seedlings affected by FeNPs under 10% PEG stress

图6 10% PEG胁迫条件下FeNPs对棉花叶片抗氧化物酶活性和MDA含量的影响注：图中不同小写字母表示差异显著（P<0.05）。

Fig. 6 Effects of FeNPs on antioxidant activity and MDA content in cotton leaves under 10% PEG stressNote：Different letters in the figure mean significant difference at P<0.05 level.

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