关键词: 纤维素降解菌, 白星花金龟幼虫, 纤维单胞菌, 基因组

Abstract: There are abundant ellulose degrading bacteria in intestinal tract of larvae of Protaetia brevitarsis and they are capable of digesting and utilizing a large amount of lignocellulose. By using the Congo red staining method and filter paper degradation methods, Cellulomonas sp. strain h9 with strong cellulose degrading ability was isolated and identified by 16S rRNA analysis. The activity of the bacteria and its related enzymes were studied under different culture conditions, the results showed that strain h9 reached the stable growth state period after 120 h fermentation in liquid CMC-Na medium, and the maximum CMC enzyme activity was 0.19 U·mL^-1. The complete genome sequence of the strain h9 was obtained by genomic de novo sequencing, and the BLAST software was used to conduct a comparison analysis with the protein sequences in COG and CAZy databases. Key genes and pathways involved in the cellulose degradation, such as β-endoglucanases, β-glucosidases, and exoglucanases, were indentified. This study  provided the theoretical basis and experimental material for cellulose degrading enzymes and constructing cellulose degrading engineering bacteria.

Key words: cellulosic degrading bacteria, Protaetia brevitarsis larva, Cellulomonas sp., genome