关键词: 生物炭, 烤烟, 微生物, 碳代谢, Biolog-ECO

Abstract: To explore the influence of different biochar dosages on the carbon metabolism of rhizospheric soil microorganism community of flue-cured tobacco, and clarify the appropriate biochar dosage, this study took Yunyan 87 as experimental material and established a contrast group(CK) with no application of biochar and 3 treatments with biochar doses as 3 000 kg/hm2（T1）, 3 750 kg/hm2（T2）, and 4 500 kg/hm2（T3）, respectively. The soil samples were collected during the fast growing period of flue-cured tobacco, and the functional diversity of rhizospheric soil microorganism community of flue-cured tobacco were analyzed by Biolog-ECO microplate technology. The results showed that: The biochar application could increase the AWCD value and various biodiversity indexes of soil microorganism, and significantly increase the utilization of carboxylic acids and polymer carbon source. T2 utilization of these 2 carbon resource categories were increased than that of the CK by 24.82% and 70.71%, respectively. The analysis of principal components indicated that carbohydrates, carboxylic acids and amino acids were the relevant carbon resources causing more differences in carbon metabolism features of soil microorganism. In conclusion, T2 showed the best capability in utilizing carbon resources, and the optimum biochar dosage was 3 750 kg/hm2.

Key words: biochar, flue-cured tobacco, microbial, carbon metabolism, Biolog-ECO