关键词: 草甘膦抗性, 鉴定, 生长特性, 泛菌属

Abstract: A 400 mmol/L glyphosate-resistant strain S1536 was isolated from the glyphosate polluted soil. The strain grew well in the medium with glyphosate concentration between 100～400 mmol/L. The optimal pH was 6.0, optimal growth temperature was 30℃, and had resistance to ampicillin. Taking S1536 genomic DNA as a template, this experiment carried out amplification and sequencing and gained 16S rDNA sequence, which registration number was MG519831 in GenBank. Compared with the published nucleotide sequence of 16S rDNA in NCBI (National Center for Biotechnology Information), S1536 showed 99% homology with Pantoea sp. Multiple sequence comparison between S1536 and the model bacteria of Pantoeasp of 16S rDNA was conducted by ClustalW. This strain showed high identity with Pantoea rodasii, reaching 99.2%, so it was named as Pantoea rodasii S1536. The strain possessed higher glyphosate resistance and good adaptability to temperature and pH. It was a superior glyphosate tolerant strain. aroA sequence resistant to herbicide was obtained through whole genome sequencing and gene annotation, which provided a solid foundation for further identification of gene function and exploitation of the molecular mechanism of high glyphosate resistance of Pantoea rodasii S1536.

Key words: glyphosate resistance, identification, growth characteristics, Pantoea sp.