关键词: 陆地棉, GhHAK1, 克隆, 低钾胁迫

Abstract: Cotton is an important economic crop typically with preference for potassium. Its yield and quality are closely related to potassium content. In this study, open read frame of GhHAK1 was cloned from the upland cotton. The dendrograms of GhHAK1 showed that it belonged to theⅠsubtribe, which was considered to be an important component of high-affinity system for K+. The trans-membrane region prediction reveals that the protein coded by this gene with 12 trans-membrane domains (TMS), and it had a long cytoplasmic ring between the second and third TMS. The sub-cellular localization experiment of onion epidermis showed that GhHAK1 was located on plasma membrane. And under the condition of low potassium (0.05 mmol/L) condition, the wild-type Arabidopsis showed obvious symptoms of K deficiency, the leaves turned yellow and bolting was obviously inhibited. But the transgenic plants growth was not affected. Besides, the content of K+ in transgenic Arabidopsis leaves was double that of the wild type and the content of K+ in root was about 1.5 times. The results showed that GhHAK1 might possess efficient potassium absorption function and would provide important genetic resources for further breeding new cotton varieties adapted to potassium deficiency soil and saline environment.

Key words: cotton (Gossypium hirsutum L.), GhHAK1, clone, low potassium stress