关键词: 北柴胡, 壳聚糖, 茉莉酸甲酯, 种子萌发

Abstract: In order to  promot seed germination of Buplerurum chinense DC in field cultivation, the seeds were used as materials and soaked in mixed liquor with different ratio of CS and MeJA for 24 h. Seeds soaked in pure water were taken as the CK. Then moisture content, volum, germination rate, soluble sugar and soluble protein contents, and free amino acids contents of the seeds were measured and analyzed. The results showed that all MeJA and CS mixed liquor could promote seed germination rate. At 35 d,  the germination rates of all treatments were significantly higher than that of the CK. The germination rates of all treatments were higher than the CK significantly. But there was no significant difference between T3 and T4, and there was significant difference between them and the other treatments. The relative maximum change rate of moisture content of T2 was the biggest, and that of T3 and the CK were the least. Except T3 and the CK, there were significant differences among the other treatments. The volume of all treatments showed first down and then up trend. T4 had the biggest volume. The total soluble sugar content showed down-up-down-up wave change trend. During seed germination process, the overall soluble protein contents showed decreasing tendency. After 35 d, the amino acid contents of the CK and T3 were higher than before, while that of T1, T2 and T4 were lower than the initial stage. Comprehensive evaluation by subordination function analysis showed that soaking seed with the mixture of 10 mg/mL CS and 224.3 μg/mL MeJA had the best effect. The germination rate could reach 54%. These results provided reference for promoting seed germination in aritificial cultivation of Bupleum chinense.

Key words: Bupleurum chinense DC., chitosan(CS), methyl jasmonate(MeJA), seed germination